INCREASING CASPASE3 GENE EXPRESSION IN MCF7 BREAST CANCER CELL LINE BY NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT

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MOSHTAGHI FATEME; ESMAEILZADEH BAHABADI SEDIGHEH; MAZAHERI MAHTA; NAJAFI SHAHLA; DAHMARDEH GHALENOO FATEME

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2016 | Volume:24 | Issue:1 Page(s): 1-11

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Title

INCREASING CASPASE3 GENE EXPRESSION IN MCF7 BREAST CANCER CELL LINE BY NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT

Author(s)

Keywords

BREAST CANCERQ1

CASPASE-3Q2

NIGELLA SATIVAQ1

Abstract

Introduction: Problems related to cancer treatment such as treatment failure, drug resistance, heavy costs, etc. have attracted the interest of many researchers to the medicinal herbs, since they have fewer side effects than chemical drugs. The findings of different studies have demonstrated that although NIGELLA SATIVA has anticancer effects, molecular mechanism of its effect on cell death of cancer cells is not yet well-recognized.Therefore, the present study aimed to explore the effect ofNigella Sativa on the viability of MCF7 BREAST CANCER cell line as well as caspase3 gene expression.Methods: In this study, MCF7 cell lines were treated with different concentrations of hydro alcoholic extracts of NIGELLA SATIVA (0, 200, 400 mg/mL) for 24, 48 and 72 h. Effects of the extract on cell viability and CASPASE-3 gene expression were analyzed by trypan blue staining and Real time PCR, respectively.Results: The results of the current study revealed that cell viability significantly decreased in a dose and time dependent manner compared to the controls (p<0.05). Real time PCR results showed that the expression of CASPASE-3 under different concentrations of extract (200 and 400 mg/ml) significantly increased compared to the control group (p<0.05).Conclusion: NIGELLA SATIVAseems to cause cell death via increasing CASPASE-3 gene expression.

Cites

THE EFFECT OF ENDURANCE TRAINING ON TUMOR TISSUE LEVELS OF CASPASE-3 AND CASPASE-9 IN MICE WITH BREAST CANCER

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APA: Copy

MOSHTAGHI, FATEME, ESMAEILZADEH BAHABADI, SEDIGHEH, MAZAHERI, MAHTA, NAJAFI, SHAHLA, & DAHMARDEH GHALENOO, FATEME. (2016). INCREASING CASPASE3 GENE EXPRESSION IN MCF7 BREAST CANCER CELL LINE BY NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 24(1), 1-11. SID. https://sid.ir/paper/36462/en

Vancouver: Copy

MOSHTAGHI FATEME, ESMAEILZADEH BAHABADI SEDIGHEH, MAZAHERI MAHTA, NAJAFI SHAHLA, DAHMARDEH GHALENOO FATEME. INCREASING CASPASE3 GENE EXPRESSION IN MCF7 BREAST CANCER CELL LINE BY NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;24(1):1-11. Available from: https://sid.ir/paper/36462/en

IEEE: Copy

FATEME MOSHTAGHI, SEDIGHEH ESMAEILZADEH BAHABADI, MAHTA MAZAHERI, SHAHLA NAJAFI, and FATEME DAHMARDEH GHALENOO, “INCREASING CASPASE3 GENE EXPRESSION IN MCF7 BREAST CANCER CELL LINE BY NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 24, no. 1, pp. 1–11, 2016, [Online]. Available: https://sid.ir/paper/36462/en

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