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Title

MiRNA-101 transfection and its effect on the cytotoxicity induction and expression of ubiquitin ligase HECTH9 in acute myeloid leukemia cells (AML)

Pages

  64-76

Abstract

 Introdution: In the present study, Lipofactamine 2000 was used as a cationic liposome for miR-101 transfection in order to investigate its cytotoxicity and its effect on the expression of ubiquitin ligase HECTH9 in acute myeloid leukemia cells (AML). Methods: miR-101 was transferred to KG-1 cells (myeloid cells) and HBMF-SPH (healthy bone marrow cells) using lipofectamine 2000 as a nano carrier. Then, using the MTT test, the 48-hour cell toxicity in both cell lines was evaluated. The effect of this miRNA on the expression of HECTH9 gene (ubiquitin Ligaase E3) was evaluated using qRT-PCR technique. Results: The findings of this study showed that Lipofactamine alone was not toxic to any of the cell lines, but lipofectamine-containing miR-101 (Lipo / miR-101) in KG-1 cells produced the highest toxicity compared to other treatments. The results of qRT-PCR test showed that Lipo / miR-101 treatment in KG-1 cells caused the highest expression in HECTH9 gene at the mRNA level. Conclusion: Lipofactamine, as a cationic liposome, can effectively transfect miR-101 into the cells and can cause miR-101 to specifically display its antitumor effect by increasing the expression of HECTH9 and regulating pathways of mitochondrial apoptotic pathway. Therefore, miR-101 can be used as a potent tumor suppressor and an effective therapeutic agent for gene therapy in the patients with AML.

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    APA: Copy

    NIKOONAHAD LOTFABADI, NARGES, MOHSENI KOUCHESFEHANI, HOMA, Sheikhha, Mohammad Hassan, & KALANTAR, SEYED MEHDI. (2018). MiRNA-101 transfection and its effect on the cytotoxicity induction and expression of ubiquitin ligase HECTH9 in acute myeloid leukemia cells (AML). JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 26(1 ), 64-76. SID. https://sid.ir/paper/36509/en

    Vancouver: Copy

    NIKOONAHAD LOTFABADI NARGES, MOHSENI KOUCHESFEHANI HOMA, Sheikhha Mohammad Hassan, KALANTAR SEYED MEHDI. MiRNA-101 transfection and its effect on the cytotoxicity induction and expression of ubiquitin ligase HECTH9 in acute myeloid leukemia cells (AML). JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;26(1 ):64-76. Available from: https://sid.ir/paper/36509/en

    IEEE: Copy

    NARGES NIKOONAHAD LOTFABADI, HOMA MOHSENI KOUCHESFEHANI, Mohammad Hassan Sheikhha, and SEYED MEHDI KALANTAR, “MiRNA-101 transfection and its effect on the cytotoxicity induction and expression of ubiquitin ligase HECTH9 in acute myeloid leukemia cells (AML),” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 1 , pp. 64–76, 2018, [Online]. Available: https://sid.ir/paper/36509/en

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