Isolation and culture of human endometrial derived cells as an in vitro model for future implantation studies

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AKYASH FATEMEH; Javidpou Mahdieh; AFLATOONIAN ABBAS; AFLATOONIAN BEHROUZ

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Paper Information

Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2019 | Volume:27 | Issue:5 Page(s): 1584-1590

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Title

Isolation and culture of human endometrial derived cells as an in vitro model for future implantation studies

Author(s)

AKYASH FATEMEH | Javidpou Mahdieh | AFLATOONIAN ABBAS | AFLATOONIAN BEHROUZ | Issue Writer Certificate

Keywords

Cell TherapyQ1

Endometrial Mesenchymal Stem/ Stromal CellsQ2

Regenerative MedicineQ1

UterusQ1

Abstract

Introduction: Monthly regeneration of endometrium after cyclical mensturation confirmed the ability of specific population of the cells that presence in the basalis layer and undergone consecutive hormonal changes that could prepared the endometrial layer for probable implantation. These cells, known as, stem cell. The aim of this study was the isolation and culture of human endometrial derived mesenchymal stem/stromal cells (EnMSCs). Methods: . In this study, human endometrial tissues were collected after fully-informed patient consent, which attended the Research and Clinical Center for Infertility. After washing and enzymatic treatment, EnMSCs were isolated and cultured in vitro. Results: Cells from endometrium were successfully isolated using enzymatically treatment and mechanically dissociation, then cultured and expanded for several passages for further characterization and evaluations. Endometrium derived cells were morphologically similar to mesenchymal stem/stromal cells (MSCs). Conclusion: The results of the present study confirm previous reports in vitro studies for the isolation and culture of human EnMSCs. Endometrial tissue is a part of Uterus with available source of MSCs with selfrenewal and differentiation capacity that undergoes a cyclical regeneration every month in normal women’ s life span. In this regard, human EnMSCs could be used for future novel therapeutic methods in Regenerative Medicine for treatment of uterine-factor infertile patients, which can lead to recurrent pregnancy loss (RPL) and finally resolve of surrogacy problems.

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APA: Copy

AKYASH, FATEMEH, Javidpou, Mahdieh, AFLATOONIAN, ABBAS, & AFLATOONIAN, BEHROUZ. (2019). Isolation and culture of human endometrial derived cells as an in vitro model for future implantation studies. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 27(5 ), 1584-1590. SID. https://sid.ir/paper/36625/en

Vancouver: Copy

AKYASH FATEMEH, Javidpou Mahdieh, AFLATOONIAN ABBAS, AFLATOONIAN BEHROUZ. Isolation and culture of human endometrial derived cells as an in vitro model for future implantation studies. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;27(5 ):1584-1590. Available from: https://sid.ir/paper/36625/en

IEEE: Copy

FATEMEH AKYASH, Mahdieh Javidpou, ABBAS AFLATOONIAN, and BEHROUZ AFLATOONIAN, “Isolation and culture of human endometrial derived cells as an in vitro model for future implantation studies,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 27, no. 5 , pp. 1584–1590, 2019, [Online]. Available: https://sid.ir/paper/36625/en

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