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Information Journal Paper

Title

Programmed cell death regulatory genes in primitive Euometazoa

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Abstract

 The aim of this manuscript is investigation, extraction and expression pattern evaluation of lifeguard genes in fresh water polyp Hydra vulgaris. For this purpose, about 100 freshwater polyps were cultured and total DNA extraction was performed. Specific primers were designed for all genes and PCR reaction applied to amplify Lifeguard genes. In order to investigate the gene expression pattern, in situ hybridization experiment used. The experiments revealed that there are three lifeguard genes in hydra, which are called Lifeguard-4 and invertebrates’ Lifeguard-1. in situ hybridization shows that Lifeguard-4 expressed in endodermic layer of polyps in all budding stages and also in different stages of oogenesis. In Conclusion, Phylogenetic tree indicates that lifeguard genes are well conserved and present at the base of Euometazoa like freshwater Hydra polyp. Since Apoptosis signaling pathway is present during Hydra gametogenesis (oogenesis and spermatogenesis), expressions of Lifeguard-4 as an Apoptosis regulatory gene, in places were Apoptosis occur is logistic and confirms the regulatory function of these genes.

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    APA: Copy

    MOTAMEDI, M.. (2019). Programmed cell death regulatory genes in primitive Euometazoa. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 31(4 ), 0-0. SID. https://sid.ir/paper/367103/en

    Vancouver: Copy

    MOTAMEDI M.. Programmed cell death regulatory genes in primitive Euometazoa. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;31(4 ):0-0. Available from: https://sid.ir/paper/367103/en

    IEEE: Copy

    M. MOTAMEDI, “Programmed cell death regulatory genes in primitive Euometazoa,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 31, no. 4 , pp. 0–0, 2019, [Online]. Available: https://sid.ir/paper/367103/en

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