Genomic polymorphism of enterococcus faecalis isolated from clinical cases using two methods of ERIC-PCR and BOX-PCR

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AHMADI FATEMEH; SIASI ELHAM; AMINI KIOMARS

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Journal: MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:41 | Issue:5 Page(s): 16-24

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Title

Genomic polymorphism of enterococcus faecalis isolated from clinical cases using two methods of ERIC-PCR and BOX-PCR

Author(s)

AHMADI FATEMEH | SIASI ELHAM | AMINI KIOMARS | Issue Writer Certificate

Keywords

Enterococcus FaecalisQ2

BOX-PCRQ1

ERIC-PCRQ1

PolymorphismQ2

Abstract

Background: The genome of Enterococcus has a large number of repetitive sequences that are randomly distributed over DNA. In ERIC-PCR, a separate pattern is obtained for each strain and is considered a separate type. Prokaryotic and eukaryotic genomes include dispersed repeat sequences that are relatively short non-coding, and dispersed in the bacterial genome. BOX-PCR and ERIC-PCR primers are complementary to these repeat sequences and allow for dedicated binding and unique BOX-PCR fingerprint patterns and ERIC-PCR with reproducibility capability. Methods: In this cross-sectional descriptive study, based on previous studies and 95% confidence level using the formula n = z2P (1-P)/d2 and acceptable error 0. 05, total of 60 Enterococcal Faecalis strains were cultured from sterile specimens on KF agar medium and incubated at 37° C for 24h and were identified by biochemical tests of suspected Enterococcal Faecalis. DNA samples were extracted and BOX-PCR and ERIC-PCR tests were performed. Results: All strains were distinguished in 25 distinct clusters at the level of similarity of 58%. Also, by analyzing the ERIC-PCR results, all strains were segregated into 15 separate clusters at a similar level of 58%. Conclusion: Molecular fingerprinting with BOX-PCR has a better subtraction and differentiation than ERIC-PCR for typing bacterial isolates and is widely used in epidemiological studies and trace source of infection and taxonomy.

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APA: Copy

AHMADI, FATEMEH, SIASI, ELHAM, & AMINI, KIOMARS. (2019). Genomic polymorphism of enterococcus faecalis isolated from clinical cases using two methods of ERIC-PCR and BOX-PCR. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 41(5 ), 16-24. SID. https://sid.ir/paper/367119/en

Vancouver: Copy

AHMADI FATEMEH, SIASI ELHAM, AMINI KIOMARS. Genomic polymorphism of enterococcus faecalis isolated from clinical cases using two methods of ERIC-PCR and BOX-PCR. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;41(5 ):16-24. Available from: https://sid.ir/paper/367119/en

IEEE: Copy

FATEMEH AHMADI, ELHAM SIASI, and KIOMARS AMINI, “Genomic polymorphism of enterococcus faecalis isolated from clinical cases using two methods of ERIC-PCR and BOX-PCR,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 41, no. 5 , pp. 16–24, 2019, [Online]. Available: https://sid.ir/paper/367119/en

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