EVALUATION OF THE EFFECTS OF INCUBATION TEMPERATURE AND PH ON THE SUSCEPTIBILITY OF CANDIDA ALBICANS ISOLATES TO KETOCONAZOLE INVITRO

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ZARIN FAR H.; YADGARI M.H.; FARAHNEJAD Z.; RIAZIPOUR M.; KATIRAEI F.

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2006 | Volume:14 | Issue:3 Page(s): 50-55

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Title

EVALUATION OF THE EFFECTS OF INCUBATION TEMPERATURE AND PH ON THE SUSCEPTIBILITY OF CANDIDA ALBICANS ISOLATES TO KETOCONAZOLE INVITRO

Author(s)

Keywords

CANDIDA ALBICANSQ2

KETOCONAZOLEQ3

VULVOVAGINITISQ3

TEMPERATUREQ2

PHQ3

Abstract

Introduction: Candidiasis, as an opportunistic infection, is caused by the Candida species. Although CANDIDA ALBICANS is classified in the body as an endogenic flora, it plays an important role in creating Candida related diseases. Candida VULVOVAGINITIS in pregnant women, diabetes mellitus patients and those using multiple antibiotics and contraceptive drugs demonstrates the high resistance of the organism against conventional medication. On the other hand, recurrent vaginitis disintegrates the long-term process of treatment in majority of the patients. The present research was done with the aim of determining the optimum conditions for susceptibility testing before retreatment of patients.Methods: 10 isolates of CANDIDA ALBICANS obtained from 31 suspected patients suffering from recurrent Candida vaginitis were incubated with KETOCONAZOLE at two PH of 7.2 and 5.5 and two TEMPERATUREs of 35ºC and 27ºC. The Microdilution broth test technique was used. The RPMI 1640 medium within the 96 well microplates with range of 12 tests was used to determine the MIC50 , MIC90 and MFC of the drug.Results: The obtained MIC50, MIC90 and MFC for KETOCONAZOLE at these conditions (T=35ºC and PH=7.2) were 0.25 to 1 μg/ml, 1 to 4 μg/ml and 64 to ≥ 512 μg/ml respectively, while these values at 27ºC, PH 5.5 were 1 to 8 μg/ml, 8 to 64 μg/ml and 512 to ≥ 512 μg/ml, at 35ºC and PH 5.5 the values were 1 to 8 μg/ml, 4 to 32 μg/ml, 256 to ≥ 512 μg/ml, while at 27ºC and PH 7.2 the values were 1 to 2 μg/ml, 8 to 32 μg/ml, 128 to ≥ 512 μg/ml, respectively.Conclusion: The obtained results confirmed that conditions with TEMPERATURE of 35ºC and PH 7.2 resulted in better treatment outcomes than other conditions.

Cites

IN VITRO ANTIFUNGAL ACTIVITY OF CLOTRIMAZOLE, MICONAZOLE AND KETOCONAZOL BY BINARY MIXTURE PATTERN AGAINST HOSPITAL ISOLATES OF CANDIDA

References

LABORATORY STUDY OF 3 CASES OF MYCOTIC KERATITIS MEMBERS

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APA: Copy

ZARIN FAR, H., YADGARI, M.H., FARAHNEJAD, Z., RIAZIPOUR, M., & KATIRAEI, F.. (2006). EVALUATION OF THE EFFECTS OF INCUBATION TEMPERATURE AND PH ON THE SUSCEPTIBILITY OF CANDIDA ALBICANS ISOLATES TO KETOCONAZOLE INVITRO. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 14(3), 50-55. SID. https://sid.ir/paper/36797/en

Vancouver: Copy

ZARIN FAR H., YADGARI M.H., FARAHNEJAD Z., RIAZIPOUR M., KATIRAEI F.. EVALUATION OF THE EFFECTS OF INCUBATION TEMPERATURE AND PH ON THE SUSCEPTIBILITY OF CANDIDA ALBICANS ISOLATES TO KETOCONAZOLE INVITRO. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2006;14(3):50-55. Available from: https://sid.ir/paper/36797/en

IEEE: Copy

H. ZARIN FAR, M.H. YADGARI, Z. FARAHNEJAD, M. RIAZIPOUR, and F. KATIRAEI, “EVALUATION OF THE EFFECTS OF INCUBATION TEMPERATURE AND PH ON THE SUSCEPTIBILITY OF CANDIDA ALBICANS ISOLATES TO KETOCONAZOLE INVITRO,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 14, no. 3, pp. 50–55, 2006, [Online]. Available: https://sid.ir/paper/36797/en

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