NASAL IMMUNOGENICITY INDUCED BY STXB AND STXB-IPAD ANTIGENS IN LABORATORY RATS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BARANVAND MAHDI; HONARI HOSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Koomesh Year:2015 | Volume:16 | Issue:3 (55) Page(s): 397-403

Download Full-Text

Persian Verion

View:

882

Download:

Cites:

Information Journal Paper

Title

NASAL IMMUNOGENICITY INDUCED BY STXB AND STXB-IPAD ANTIGENS IN LABORATORY RATS

Author(s)

BARANVAND MAHDI | HONARI HOSEIN | Issue Writer Certificate

Keywords

SHIGELLA DYSENTERIAEQ2

ESCHERICHIA COLI O157Q2

SHIGA TOXIN 2Q2

RATSQ2

Abstract

Introduction: Shigella and E. coli bacteria are the most common cause of Diarrhea, though as yet no effective vaccine against them has been produced. IpaD protein plays an important role in invasion and infection caused by Shigella. Another major virulence factor in SHIGELLA DYSENTERIAE type 1 and E. coli O157: H7 is Shigella enterotoxin or (STxB). IpaD protein in combination with STxB can produce a suitable candidate vaccine. In this study, the nasal STxB and STxB-IpaD fused recombinant proteins antibody titers and their immunogenicity were assessed and compared in RATS.Materials and Methods: (pET28- stxB) and (pET28-ipaD-stxB) vectors were prepared at Biology center of the Imam Hussein University (AS). Transformation of these plasmids into E. coli BL21 DE3 bacteria were confirmed by PCR and enzymatic digestion. Production of recombinant proteins were confirmed by SDS-PAGE and Western blotting. Expression of fused STxB-IpaD and STxB antigens were performed under induction of IPTG. After protein purifications, using affinity chromatography, antigens were prescribed nasally to five groups of RATS in four consecutive sessions. Later the polyclonal antibodies produced in rat sera were measured.Results: ELISA showed that antibody titers were increased by the combination of IpaD to STxB compared to that produced against single STxB antigen. The immunized RATS with StxB antigen were able to tolerate up to six fold of LD50, while RATS that were immunized with STxB-IpaD combined antigens were able tolerate up to tenfold of LD50 for E. coli O157: H7 Shiga toxin.Conclusion: The protein produced from the fusion of ipaD and stxB genes, can increase the effect of single STxB antigen immunogenicity. Recombinant proteins fused with STxB protein without chemicals adjuvants can be recommended in the form of nasal drops as possible candidates for vaccines against E. coli and Shigella types.

Cites

No record.

References

No record.

Cite

APA: Copy

BARANVAND, MAHDI, & HONARI, HOSEIN. (2015). NASAL IMMUNOGENICITY INDUCED BY STXB AND STXB-IPAD ANTIGENS IN LABORATORY RATS. KOOMESH, 16(3 (55)), 397-403. SID. https://sid.ir/paper/36808/en

Vancouver: Copy

BARANVAND MAHDI, HONARI HOSEIN. NASAL IMMUNOGENICITY INDUCED BY STXB AND STXB-IPAD ANTIGENS IN LABORATORY RATS. KOOMESH[Internet]. 2015;16(3 (55)):397-403. Available from: https://sid.ir/paper/36808/en

IEEE: Copy

MAHDI BARANVAND, and HOSEIN HONARI, “NASAL IMMUNOGENICITY INDUCED BY STXB AND STXB-IPAD ANTIGENS IN LABORATORY RATS,” KOOMESH, vol. 16, no. 3 (55), pp. 397–403, 2015, [Online]. Available: https://sid.ir/paper/36808/en

Related Journal Papers