Evaluation of Immunity Level of B. C. G Vaccine Against Tuberculosis Using Skin Test for Tuberculin and in Vitro Response of Lymphocytes to B. C. G Antigens in Shavoor City Students

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Abdolkhani Sakineh; MAHMOUDI HASAN; SHEIKHI ABDOLKARIM; ROAYAEI ARDAKANI MOHAMMAD

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Journal: Jundishapur Scientific Medical Journal Year:2019 | Volume:18 | Issue:2 Page(s): 205-213

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Title

Evaluation of Immunity Level of B. C. G Vaccine Against Tuberculosis Using Skin Test for Tuberculin and in Vitro Response of Lymphocytes to B. C. G Antigens in Shavoor City Students

Author(s)

Abdolkhani Sakineh | MAHMOUDI HASAN | SHEIKHI ABDOLKARIM | ROAYAEI ARDAKANI MOHAMMAD | Issue Writer Certificate

Keywords

Mycobacterium tuberculosisQ2

Interferon gammaQ2

BCG vaccineQ1

Abstract

Background and Objectives: Tuberculin skin test is a screening test for tuberculosis. The aim of this investigation studied in vitro the immunity level out of Bacillus Calmette– Guerin (BCG) vaccine, through measurement of Interferon gamma (IFN γ ) in growth medium, in the students of Shavoor city. Subjects and Methods: This research is an experimental study that was performed on 32 male and female students of the three educational levels. After blood sampling, the peripheral blood mononuclear cells were isolated by Ficoll solution and 2×105 Cell/Wells with 5 or 20 μ l B. C. G vaccine were co-cultured in CO2 incubator in 37° C for 48 and 72 hours, and IFN γ level measured by the ELIZA method. For statistical analyses, independent t-test was used to analyze the differences between the two groups using SPSS software version 23. Results: Analysis of the results showed that there was a significant difference between the size of the induration at different ages of 13 years old (P<0. 05). Also, there was no significant difference between the size of the induration in boys and girls (P>0. 05). There was a significant difference between the age, time, and amounts of the bacterium 5 or 20 μ L of the B. C. G vaccine used with the level of IFN γ secretion in the two groups (P <0. 05). Conclusion: With regard to the results, IFN γ secretion is directly related to the dose of bacteria used and the monocular blood cells of the students and incubation time. Also, the level of IFN γ secretion at younger ages was lower than that at an early age.

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References

Evaluation of TNF-α cytokine production in patients with tuberculosis compared to healthy people

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APA: Copy

Abdolkhani, Sakineh, MAHMOUDI, HASAN, SHEIKHI, ABDOLKARIM, & ROAYAEI ARDAKANI, MOHAMMAD. (2019). Evaluation of Immunity Level of B. C. G Vaccine Against Tuberculosis Using Skin Test for Tuberculin and in Vitro Response of Lymphocytes to B. C. G Antigens in Shavoor City Students. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 18(2 ), 205-213. SID. https://sid.ir/paper/369039/en

Vancouver: Copy

Abdolkhani Sakineh, MAHMOUDI HASAN, SHEIKHI ABDOLKARIM, ROAYAEI ARDAKANI MOHAMMAD. Evaluation of Immunity Level of B. C. G Vaccine Against Tuberculosis Using Skin Test for Tuberculin and in Vitro Response of Lymphocytes to B. C. G Antigens in Shavoor City Students. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2019;18(2 ):205-213. Available from: https://sid.ir/paper/369039/en

IEEE: Copy

Sakineh Abdolkhani, HASAN MAHMOUDI, ABDOLKARIM SHEIKHI, and MOHAMMAD ROAYAEI ARDAKANI, “Evaluation of Immunity Level of B. C. G Vaccine Against Tuberculosis Using Skin Test for Tuberculin and in Vitro Response of Lymphocytes to B. C. G Antigens in Shavoor City Students,” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 18, no. 2 , pp. 205–213, 2019, [Online]. Available: https://sid.ir/paper/369039/en

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