SUB-CLONING OF ALPHA SUBUNIT OF ANTERIOR PITUITARY GLYCOPROTEIN HORMONE INTO A MAMMALIAN EXPRESSION VECTOR

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AKBARI EIDGAHI MOHAMMAD REZA; NASR REZA; SHAEBANI ALI AKBAR; GHADAMYARI MOJTABA

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Journal: Koomesh Year:2012 | Volume:13 | Issue:2 (42) Page(s): 143-150

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Title

SUB-CLONING OF ALPHA SUBUNIT OF ANTERIOR PITUITARY GLYCOPROTEIN HORMONE INTO A MAMMALIAN EXPRESSION VECTOR

Author(s)

AKBARI EIDGAHI MOHAMMAD REZA | NASR REZA | SHAEBANI ALI AKBAR | GHADAMYARI MOJTABA | Issue Writer Certificate

Keywords

FOLLICLE STIMULATING HORMONEQ2

PITUITARY HORMONESQ2

GLYCOPROTEIN HORMONESQ2

ALPHA SUBUNITQ2

MAMMALSQ2

Abstract

Introduction: Anterior pituitary GLYCOPROTEIN HORMONES include thyroid stimulating hormone, luteinizing hormone, FOLLICLE STIMULATING HORMONE, and gonadotropin hormone. Each of them contains alpha and beta subunits. The ALPHA SUBUNIT gene is the same in all of these hormones and contains 4 exons and 3 introns.The beta subunit is responsible for specific function of each hormone. The aim of this study was to clone alpha chain cDNA of GLYCOPROTEIN HORMONES in a proper vector for eukaryotic system.Material and Methods: To clone cDNA, ALPHA SUBUNIT of GLYCOPROTEIN HORMONES was amplified by using one pair primers and T.vector as template and cloned in Not I and Bam HI sites of pcDNA3.1 plasmid. The recombinant plasmid transformed to E.coli Top 10F¢ cell and colonies that contain plasmid were selected by Colony PCR. The accuracy of extracted plasmid of these clones was approved by enzyme digestion and sequencing.Results: Enzyme analysis showed that pcDNA3.1-F351a had correct structure and sequencing confirmed by 100% homology of the gene with reported alpha gene in Gene Bank.Conclusion: Because of its proper structure, this plasmid is able to transform to Eukaryotic system and translation.

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APA: Copy

AKBARI EIDGAHI, MOHAMMAD REZA, NASR, REZA, SHAEBANI, ALI AKBAR, & GHADAMYARI, MOJTABA. (2012). SUB-CLONING OF ALPHA SUBUNIT OF ANTERIOR PITUITARY GLYCOPROTEIN HORMONE INTO A MAMMALIAN EXPRESSION VECTOR. KOOMESH, 13(2 (42)), 143-150. SID. https://sid.ir/paper/36929/en

Vancouver: Copy

AKBARI EIDGAHI MOHAMMAD REZA, NASR REZA, SHAEBANI ALI AKBAR, GHADAMYARI MOJTABA. SUB-CLONING OF ALPHA SUBUNIT OF ANTERIOR PITUITARY GLYCOPROTEIN HORMONE INTO A MAMMALIAN EXPRESSION VECTOR. KOOMESH[Internet]. 2012;13(2 (42)):143-150. Available from: https://sid.ir/paper/36929/en

IEEE: Copy

MOHAMMAD REZA AKBARI EIDGAHI, REZA NASR, ALI AKBAR SHAEBANI, and MOJTABA GHADAMYARI, “SUB-CLONING OF ALPHA SUBUNIT OF ANTERIOR PITUITARY GLYCOPROTEIN HORMONE INTO A MAMMALIAN EXPRESSION VECTOR,” KOOMESH, vol. 13, no. 2 (42), pp. 143–150, 2012, [Online]. Available: https://sid.ir/paper/36929/en

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