Investigation of the effect of light, phenylalanine and caffeic acid on the production of phenolic acids in cell suspension culture of Salvia leriifolia

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MODARRES MASOOMEH; Ghasimi Hagh Ziba

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Journal: JOURNAL OF PLANT PROCESS AND FUNCTION Year:2019 | Volume:8 | Issue:29 Page(s): 171-179

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Information Journal Paper

Title

Investigation of the effect of light, phenylalanine and caffeic acid on the production of phenolic acids in cell suspension culture of Salvia leriifolia

Author(s)

MODARRES MASOOMEH | Ghasimi Hagh Ziba | Issue Writer Certificate

Keywords

Salvia leriifoliaQ1

phenolic acidsQ1

lightQ2

phenylalanineQ1

Abstract

Salvia leriifolia is a medicinal plant belonging to lamiaceae family. In this study, the influence of light, phenylalanine and caffeic acid on cell growth and accumulations of phenolic acids were investigated. For this propose, the leaf explants were cultured in MS medium supplemented with BAP and NAA for callus induction. A cell suspension culture of S. leriifolia derived from leaf-derived callus was established in liquid MS medium with the same hormonal content. The biomass and phenolic acids content were analyzed after 15 days of the culture. Quantitative analyses of the phenolic acids in ethanolic extracts from the biomass were conducted by applying the HPLC method. Both dry weight and phenolic acid content were increased by light irradiation. Addition of 10 mg/l phenylalanine enhanced accumulation of caffeic acid (4fold), rosmarinic acd(4fold) and Salvianolic acid(1. 6fold) compared to control. Addition of 10 mg/l caffeic acid decreased cell growth and accumulation of phenolic acids. This study will help us understand how secondary metabolism in Salvia leriifolia responds to precursors and light provide a reference for the improvement of the production of targeted compounds in the near future.

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APA: Copy

MODARRES, MASOOMEH, & Ghasimi Hagh, Ziba. (2019). Investigation of the effect of light, phenylalanine and caffeic acid on the production of phenolic acids in cell suspension culture of Salvia leriifolia. JOURNAL OF PLANT PROCESS AND FUNCTION, 8(29 ), 171-179. SID. https://sid.ir/paper/369643/en

Vancouver: Copy

MODARRES MASOOMEH, Ghasimi Hagh Ziba. Investigation of the effect of light, phenylalanine and caffeic acid on the production of phenolic acids in cell suspension culture of Salvia leriifolia. JOURNAL OF PLANT PROCESS AND FUNCTION[Internet]. 2019;8(29 ):171-179. Available from: https://sid.ir/paper/369643/en

IEEE: Copy

MASOOMEH MODARRES, and Ziba Ghasimi Hagh, “Investigation of the effect of light, phenylalanine and caffeic acid on the production of phenolic acids in cell suspension culture of Salvia leriifolia,” JOURNAL OF PLANT PROCESS AND FUNCTION, vol. 8, no. 29 , pp. 171–179, 2019, [Online]. Available: https://sid.ir/paper/369643/en

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