Identification of Salmonella and E. coli with invA and lamB Genes by Multiplex PCR in Chicken Samples

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SIASI ELHAM; hassanpour baghbanchian Najmeh; NOWROOZI JAMILEH

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2019 | Volume:23 | Issue:83 Page(s): 75-85

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Title

Identification of Salmonella and E. coli with invA and lamB Genes by Multiplex PCR in Chicken Samples

Author(s)

SIASI ELHAM | hassanpour baghbanchian Najmeh | NOWROOZI JAMILEH | Issue Writer Certificate

Keywords

Chicken productsQ2

E.coliQ3

SalmonellaQ2

Special geneQ1

Multiplex PCRQ2

Abstract

Background and objective: Salmonella and Escherichia coli are the most common food pathogens bacterial especially in Chicken products. Rapidly, specifically and simultaneously detection of the pathogens by identification of Special genes, is important. This study aim was identification of both E. coli and Salmonella in Chicken products by using Multiplex PCR with lamB and invA genes amplification. Materials and methods: 100 chicken samples were obtained. Samples were washed by sterile saline water and were cultured in biochemical medium. Salmonella and Escherichia coli strains were purified and DNA extraction was performed. Then amplification of both invA and lamB genes were used by Multiplex PCR. Results: From 100 chicken samples, 9 samples and 26 samples were isolated by biochemichal tests, Salmonella and E. coli, respectively. Multiplex PCR results showed ferequency of invA gene was 9% and for lamB gene was 26%, As also, frequency of both genes in samples were 1%. Conclusion: In this study, Multiplex PCR results was based on Salmonella and E. coli special virulence genes was comfirmed biochemical tests results. Therefore comparation of results showed Multiplex PCR for rapid and simultaneous detection of food pathogens has high sensitivity and can be a reliable method for controlling of food contamination.

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APA: Copy

SIASI, ELHAM, hassanpour baghbanchian, Najmeh, & NOWROOZI, JAMILEH. (2019). Identification of Salmonella and E. coli with invA and lamB Genes by Multiplex PCR in Chicken Samples. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 23(83 ), 75-85. SID. https://sid.ir/paper/370720/en

Vancouver: Copy

SIASI ELHAM, hassanpour baghbanchian Najmeh, NOWROOZI JAMILEH. Identification of Salmonella and E. coli with invA and lamB Genes by Multiplex PCR in Chicken Samples. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2019;23(83 ):75-85. Available from: https://sid.ir/paper/370720/en

IEEE: Copy

ELHAM SIASI, Najmeh hassanpour baghbanchian, and JAMILEH NOWROOZI, “Identification of Salmonella and E. coli with invA and lamB Genes by Multiplex PCR in Chicken Samples,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 23, no. 83 , pp. 75–85, 2019, [Online]. Available: https://sid.ir/paper/370720/en

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