ASSESMENT, ISOLATION AND IDENTIFICATION OF CYCLOSPORIN A BY GEL CHROMATOGRAPHY, FUNGICIDAL ACTIVITY AND SPECTROSCOPY

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GHARAVI SEYED MANOUCHEHR; KHODAI N.; BAHRI NAJAFI R.

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Journal: Koomesh Year:2002 | Volume:3 | Issue:3-4 Page(s): 115-122

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Information Journal Paper

Title

ASSESMENT, ISOLATION AND IDENTIFICATION OF CYCLOSPORIN A BY GEL CHROMATOGRAPHY, FUNGICIDAL ACTIVITY AND SPECTROSCOPY

Author(s)

GHARAVI SEYED MANOUCHEHR | KHODAI N. | BAHRI NAJAFI R. | Issue Writer Certificate

Keywords

CYCLOSPORINQ3

FERMENTATIONQ2

CHROMATOGRAPHYQ3

MUTATIONQ2

FUNGICIDAL ACTIVITYQ3

Abstract

Introduction: T.inflatum (Tolypocladium inflatum) is a primitive fungi that reproducts via conidiums. Spors grow up on malt extract culture at 6-32oC at wet or dry medium. Of course it is found of some others culture which are contain aminoacids, mineral or organic matter. CYCLOSPORIN A (Cy A) is the metabolite of t.inflatum. Cy A is an immunosuppressive drug. The aim of this study was to promote production of Cy A by MUTATION via ultraviolet radiation (UVR) and to find out a suitable medium for auxotrophs.Material and Methods: Tolypocladium intfatum (DSM 915), malt extract and sabourd 4% dextrose agar, Bactopeptamine, casein hydrolysate and yeast extract dehydrated. The fungi was cultivated for 10-20 days duration on a medium contains 2% malt extract, 4% yeast extract and 20% agar at pH 5.7. Extraction was carried out by gel CHROMATOGRAPHY. For MUTATION of the ungi, UVR was used, and for identification of Cy A fungicidal, ultraviolet, visible and infra-red pectroscopy were methods of choice.Results: White velvey colonies were collected from the medium of agar and malt extract. samples and fractions of gel CHROMATOGRAPHY extraction showed antifungal activity on aspergillus iger. Ultraviolet visible spectroscopy gave λmax similar to that of standared and infra-red spectra was similar to the standared spectra.Conclusion: Auxotrophic strains were cultivated on synthetic and raw mediums. Finally the high production of Cy A was produced of sugar factroy and hogwash of corn oil pressing factroy, at dark place.

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APA: Copy

GHARAVI, SEYED MANOUCHEHR, KHODAI, N., & BAHRI NAJAFI, R.. (2002). ASSESMENT, ISOLATION AND IDENTIFICATION OF CYCLOSPORIN A BY GEL CHROMATOGRAPHY, FUNGICIDAL ACTIVITY AND SPECTROSCOPY. KOOMESH, 3(3-4), 115-122. SID. https://sid.ir/paper/37101/en

Vancouver: Copy

GHARAVI SEYED MANOUCHEHR, KHODAI N., BAHRI NAJAFI R.. ASSESMENT, ISOLATION AND IDENTIFICATION OF CYCLOSPORIN A BY GEL CHROMATOGRAPHY, FUNGICIDAL ACTIVITY AND SPECTROSCOPY. KOOMESH[Internet]. 2002;3(3-4):115-122. Available from: https://sid.ir/paper/37101/en

IEEE: Copy

SEYED MANOUCHEHR GHARAVI, N. KHODAI, and R. BAHRI NAJAFI, “ASSESMENT, ISOLATION AND IDENTIFICATION OF CYCLOSPORIN A BY GEL CHROMATOGRAPHY, FUNGICIDAL ACTIVITY AND SPECTROSCOPY,” KOOMESH, vol. 3, no. 3-4, pp. 115–122, 2002, [Online]. Available: https://sid.ir/paper/37101/en

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