The effect of extract of Dunaliella salina L. on the expression of Bax in glioblastoma cancer cell Line

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Kavousi Mahsa; Seyed Hosseini Seyed Shamin

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Journal: JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES Year:2020 | Volume:8 | Issue:3 Page(s): 37-48

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Title

The effect of extract of Dunaliella salina L. on the expression of Bax in glioblastoma cancer cell Line

Author(s)

Kavousi Mahsa | Seyed Hosseini Seyed Shamin | Issue Writer Certificate

Keywords

Dunaliella salinaQ1

Cell Line U87Q1

MTT assayQ1

Real time PCRQ1

BaxQ1

GAPDHQ1

IC50Q1

Abstract

Background & Aim: Plant extracts can change the expression of genes in cancer cells. Up to now, no study has been performed on the effect of algae extract. The aim of this research was to investigate the changes in Bax apoptotic gene expression in glioblastoma cell line and to determine the rate of cell death in cancer cells treated with Dunalilla salina extract. U87 cell line contains glioblastoma cancer cells as the most acute type of human brain tumor. Several genes, including Bax, play important roles in initiating apoptosis and the expression of Bax decreases during cancer. Today, because of the side effects of chemical drugs, the tendency to use algae and medicinal plants in the treatment of diseases has increased. Methods: The U87 cell line was prepared from Iranian Biological Resources Center and cultivated. Cells were divided into two treatment and control groups. Dunaliella extract was affected on the treatment group with concentrations of 0. 5, 1, 2, 5, 10, 15, 20, 50 and 100 mg/ml and its toxicity was measured using MTT assay. Bax expression was examined by Real time PCR. RNA was extracted from the cells of the treatment and control group and the cDNA was made. The expression of genes was evaluated using Real time PCR and Δ Δ Ct. Results: The result of MTT indicated that IC50 was 10. 01 mg/ml for 48 hours of treatment. By using Real time PCR, it was found that in these cells, the expression level of Bax apoptotic gene increased 4-fold (p-value <0. 001). Conclusion: Treatment with algae extract increased gene expression and destroyed cancer cells. It is suggested to investigate the effect of other algae extracts on the expression of other genes involved in apoptosis.

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APA: Copy

Kavousi, Mahsa, & Seyed Hosseini, Seyed Shamin. (2020). The effect of extract of Dunaliella salina L. on the expression of Bax in glioblastoma cancer cell Line. JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES, 8(3 ), 37-48. SID. https://sid.ir/paper/371200/en

Vancouver: Copy

Kavousi Mahsa, Seyed Hosseini Seyed Shamin. The effect of extract of Dunaliella salina L. on the expression of Bax in glioblastoma cancer cell Line. JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2020;8(3 ):37-48. Available from: https://sid.ir/paper/371200/en

IEEE: Copy

Mahsa Kavousi, and Seyed Shamin Seyed Hosseini, “The effect of extract of Dunaliella salina L. on the expression of Bax in glioblastoma cancer cell Line,” JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES, vol. 8, no. 3 , pp. 37–48, 2020, [Online]. Available: https://sid.ir/paper/371200/en

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