Evaluation of P53 and MICAL-2 Gene Expression and Mutations in Gastric Cancer Patients in Kerman

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Salari Saeedeh; MAHMOODI MEHDI; TORKZADEH MAHANI MASOUD; ASKARI NAHID; Hajizadeh Mohammadreza; BANIASADI NADIEH; MALEKPOUR AFSHAR REZA; KHOSHDEL ALIREZA

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Journal: Research in Medicine Year:2019 | Volume:42 | Issue:4 Page(s): 247-253

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Title

Evaluation of P53 and MICAL-2 Gene Expression and Mutations in Gastric Cancer Patients in Kerman

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Keywords

Gastric cancerQ2

P53Q2

MICAL-2Q1

Real Time RT-qPCRQ1

PCR-SSCPQ3

KermanQ3

Abstract

Background: Stomach cancer is one of the most common cancers after lung cancer and the second cause of cancer deaths worldwide. Changes in the expression level of genes are one of the most important factors in cancers. Among them P53 and MICAL-2 were found to have changes in their expression levels. P53 gene plays an important role as a tumor suppressor along with regulating the cell cycle. MICAL-2 encodes monooxygenase which raises F-actin depolymerization. Increased expression of this gene involves the conversion of mesenchymal cells to epithelial which has been associated with metastasis. Considering that the MICAL-2 gene had not previously been studied with P53 gene at the same time in Gastric cancer, the present study was conducted to evaluate the expression level of P53 and MICAL-2 genes in stomach cancer patients in Kerman. Materials and Methods: A case-control study was designed to determine the changes in the expression level of P53 and MICAL-2 genes. A total of 30 tumor tissues and marginal non-tumor control tissues were obtained from patients. Total mRNA of the samples was extracted, and then, after cDNA synthesis, Real Time RT-qPCR, PCR-SSCP, and sequencing were performed to measure the gene expressions. T-test and ANOVA were used for data analysis. Results: The expression levels of P53 gene were 0. 38 and 0. 47 in cancerous tissues whereas they were 2. 04 and 3. 11 in normal tissues for exon 6 and 7, respectively. But the results indicated that exons 4 and 5 were not statistically different in normal and cancerous tissues. For MICAL-2, the expression level in tumor had a 2. 2-fold increase in comparison with normal tissues. Therefore, it was found that the expression levels of P53 and MICAL-2 genes were significantly higher in tumor tissues compared to those from normal tissues (P<0. 05). On the other hand, sequencing results demonstrated missense and deletion mutations in exon 4. Conclusion: Considering the changes in the expression of P53 and MICAL2 genes in Gastric cancer, changes in the expression of the genes can be used as biomarker in individuals with family history of this problem, because diagnosis of cancer in the early stages can definitely result in better response to therapies.

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APA: Copy

Salari, Saeedeh, MAHMOODI, MEHDI, TORKZADEH MAHANI, MASOUD, ASKARI, NAHID, Hajizadeh, Mohammadreza, BANIASADI, NADIEH, MALEKPOUR AFSHAR, REZA, & KHOSHDEL, ALIREZA. (2019). Evaluation of P53 and MICAL-2 Gene Expression and Mutations in Gastric Cancer Patients in Kerman. PAJOUHESH DAR PEZESHKI, 42(4 ), 247-253. SID. https://sid.ir/paper/374262/en

Vancouver: Copy

Salari Saeedeh, MAHMOODI MEHDI, TORKZADEH MAHANI MASOUD, ASKARI NAHID, Hajizadeh Mohammadreza, BANIASADI NADIEH, MALEKPOUR AFSHAR REZA, KHOSHDEL ALIREZA. Evaluation of P53 and MICAL-2 Gene Expression and Mutations in Gastric Cancer Patients in Kerman. PAJOUHESH DAR PEZESHKI[Internet]. 2019;42(4 ):247-253. Available from: https://sid.ir/paper/374262/en

IEEE: Copy

Saeedeh Salari, MEHDI MAHMOODI, MASOUD TORKZADEH MAHANI, NAHID ASKARI, Mohammadreza Hajizadeh, NADIEH BANIASADI, REZA MALEKPOUR AFSHAR, and ALIREZA KHOSHDEL, “Evaluation of P53 and MICAL-2 Gene Expression and Mutations in Gastric Cancer Patients in Kerman,” PAJOUHESH DAR PEZESHKI, vol. 42, no. 4 , pp. 247–253, 2019, [Online]. Available: https://sid.ir/paper/374262/en

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