INVESTIGATION OF RELATIONSHIP BETWEEN THE C825T POLYMORPHISM IN GNB3 GENE AND OBESITY IN THE POPULATION OF ARDABIL, IRAN

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NATEGH F.; YAGHOUBI H.; RAJABALIZADEH K.

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Journal: Journal of Molecular and Cellular Researches Year:2017 | Volume:30 | Issue:4 Page(s): 558-571

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Title

INVESTIGATION OF RELATIONSHIP BETWEEN THE C825T POLYMORPHISM IN GNB3 GENE AND OBESITY IN THE POPULATION OF ARDABIL, IRAN

Author(s)

NATEGH F. | YAGHOUBI H. | RAJABALIZADEH K. | Issue Writer Certificate

Keywords

OBESITYQ2

ARDABILQ2

POLYMORPHISM C825TQ1

GENE GNB3Q1

PCR-RFLPQ1

Abstract

OBESITY is currently a risk factor for many diseases and health problems that one of the main causes of its incidence are genetic factors. One of the genetic factors that recently considered for OBESITY is polymorphisms of GNB3. This study aimed to investigate the association between C825T polymorphism in GNB3 with OBESITY in the population of ARDABIL. In this case-control study, 100 obese subjects (case group) and 100 healthy subjects (control group) were included basis of their body mass index.5 ml of blood was taken for molecular tests from subjects. DNA of all blood samples was extracted using standard phenol chloroform procedure and its amount was measured using spectrophotometer. Primers for copying GNB3 gene sequence were designed using GNB3 sequence and Primer3 software. The obtained PCR-RFLP products electrophoresed on 2% agarose gel and by the 100volt current for 30 min along with a normal or control DNA sample and then were visualized by Ethidium bromide. The TT genotype frequency in obese individuals was significantly greater than its frequency in healthy individuals (38% versus 13%, P=0.0001). Also, the T allele frequency in obese individuals was significantly greater than its frequency in healthy individuals (60% versus 39%, P=0.0029). The results of this study showed that C825T polymorphism of GNB3 gene is associated with OBESITY in Ardebil population and can represent one of the possible genetic causes of OBESITY incidence in this area.

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APA: Copy

NATEGH, F., YAGHOUBI, H., & RAJABALIZADEH, K.. (2017). INVESTIGATION OF RELATIONSHIP BETWEEN THE C825T POLYMORPHISM IN GNB3 GENE AND OBESITY IN THE POPULATION OF ARDABIL, IRAN. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 30(4 ), 558-571. SID. https://sid.ir/paper/375849/en

Vancouver: Copy

NATEGH F., YAGHOUBI H., RAJABALIZADEH K.. INVESTIGATION OF RELATIONSHIP BETWEEN THE C825T POLYMORPHISM IN GNB3 GENE AND OBESITY IN THE POPULATION OF ARDABIL, IRAN. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2017;30(4 ):558-571. Available from: https://sid.ir/paper/375849/en

IEEE: Copy

F. NATEGH, H. YAGHOUBI, and K. RAJABALIZADEH, “INVESTIGATION OF RELATIONSHIP BETWEEN THE C825T POLYMORPHISM IN GNB3 GENE AND OBESITY IN THE POPULATION OF ARDABIL, IRAN,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 30, no. 4 , pp. 558–571, 2017, [Online]. Available: https://sid.ir/paper/375849/en

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