Immunological Evaluation of HIV-1 VLP Harboring MPER-V3 in BALB/c Mice Model

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TOHIDI F.; SADAT S.M.; BOLHASSANI A.; YAGHOBI R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2018 | Volume:21 | Issue:2 Page(s): 95-100

Download Full-Text

Persian Verion

View:

335

Download:

Cites:

Information Journal Paper

Title

Immunological Evaluation of HIV-1 VLP Harboring MPER-V3 in BALB/c Mice Model

Author(s)

TOHIDI F. | SADAT S.M. | BOLHASSANI A. | YAGHOBI R. | Issue Writer Certificate

Keywords

VaccineQ2

Nano-precipitationQ1

VLPQ2

HIV1-Q3

ImmunizationQ1

BALB/cQ1

Abstract

Aims Developing an effective Vaccine against Human Immunodeficiency Virus (HIV) is necessary. The aim of this study was the immunological evaluation of HIV-1 VLP harboring MPER-V3 in BALB/c mice model. Materials & Methods In the present experimental research, presenting 40 female mice, which were between 6 and 8 weeks old, were used for Immunization with VLP MPER-V3. The mice were divided into 8 groups and in each group, 5 mice were considered. Injections were performed three times at three weeks intervals and subcutaneously in a volume of 100μ l per mouse. Two weeks after last injection, mouse blood samples were collected by retro-orbital bleeding and immune responses were evaluated in serum for levels of total IgG and splenocytes for cytokine assay, using ELISA method. The data analysis was performed, using Mann-Whitney test and one-way analysis of variance. Findings The level of total antibody production was very high in all groups that had VLP alone or with adjuvant immunity, having a significant difference with the control group (p<0. 05). IgG1 was the predominant isotype (Th2-biased response) in groups that had VLP injections alone or with adjuvant. Conclusion VLPs can stimulate the humoral immune system in mice immunized with these particles alone or formulated with adjuvant. Also, the level of production of IL-5 in the presence of the Vaccine candidate as VLP increased significantly.

Cites

No record.

References

No record.

Cite

APA: Copy

TOHIDI, F., SADAT, S.M., BOLHASSANI, A., & YAGHOBI, R.. (2018). Immunological Evaluation of HIV-1 VLP Harboring MPER-V3 in BALB/c Mice Model. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 21(2 ), 95-100. SID. https://sid.ir/paper/376534/en

Vancouver: Copy

TOHIDI F., SADAT S.M., BOLHASSANI A., YAGHOBI R.. Immunological Evaluation of HIV-1 VLP Harboring MPER-V3 in BALB/c Mice Model. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2018;21(2 ):95-100. Available from: https://sid.ir/paper/376534/en

IEEE: Copy

F. TOHIDI, S.M. SADAT, A. BOLHASSANI, and R. YAGHOBI, “Immunological Evaluation of HIV-1 VLP Harboring MPER-V3 in BALB/c Mice Model,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 21, no. 2 , pp. 95–100, 2018, [Online]. Available: https://sid.ir/paper/376534/en

Related Journal Papers