Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin-Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method

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Rahimi Nahid; Honarmand Jahromy Sahar; ZARE KARIZI SHOHREH

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Journal: Iranian Journal of Medical Microbiology Year:2019 | Volume:13 | Issue:3 Page(s): 194-209

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Title

Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin-Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method

Author(s)

Rahimi Nahid | Honarmand Jahromy Sahar | ZARE KARIZI SHOHREH | Issue Writer Certificate

Keywords

Antibiotic susceptibilityQ1

MDRQ1

Acinetobacter baumanniiQ2

flow cytometerQ1

Abstract

Background and Aims: Flow cytometry is a rapid method that can analyze thousands of cells per second and can be used for determination of microbial populations and determination of bacterial antimicrobial susceptibility. In this study antibiotic resistance pattern of Acinetobacter baumannii isolates by flow cytometer was evaluated. Materials and Methods: 55 isolates of A. baumannii were isolated from clinical specimen of patients and were identified by biochemical tests. Antibiotic resistance patterns were studied by disc diffusion method and MDR strains were selected. MIC of Meropenem-Tazobactam and Piperacillin were determined. Also antibiotic resistance pattern of isolates was determined by coloring with Rhodamine-123 and flow cytometry. Excel software and STDV test were used to calculate the standard deviation of antibiotic resistance and MIC results. To evaluate the agreement between the results of antibiotic resistance pattern using disk diffusion methods and the MIC and Flow cytometry category agreement was used. Results: 98% of isolates were MDR. The MIC ranges for maropenem were 8-256 μ g/mL and for Piperacilllin-tazobactam were 128-1024 μ g/mL. By flow cytometry it was demonstrated that at concentrations of 8, 4 and 2 μ g/mL of meropenem, only 1. 96%, 1. 44% and 0. 59%, of cells were killed respectively. At concentrations of 64, 128 and 16 μ g/mL of piperacillin, 13. 8%, 11. 3% and 5. 9%of cells were killed respectively. Reducing the number of living bacteria was observed with increasing concentrations of both antibiotics. Conclusion: The similarity between the results of flow cytometry and both agar and broth antibacterial susceptibility methods showed flow cytometry as a reliable and rapid test that can be used for this purpose.

Cites

ارزیابی سیستم توکسین-آنتی توکسین در اسینتوباکترهای مقاوم چنددارویی Multidrug resistant جدا شده از نمونه های کلینیکی

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APA: Copy

Rahimi, Nahid, Honarmand Jahromy, Sahar, & ZARE KARIZI, SHOHREH. (2019). Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin-Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 13(3 ), 194-209. SID. https://sid.ir/paper/376839/en

Vancouver: Copy

Rahimi Nahid, Honarmand Jahromy Sahar, ZARE KARIZI SHOHREH. Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin-Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2019;13(3 ):194-209. Available from: https://sid.ir/paper/376839/en

IEEE: Copy

Nahid Rahimi, Sahar Honarmand Jahromy, and SHOHREH ZARE KARIZI, “Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin-Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 13, no. 3 , pp. 194–209, 2019, [Online]. Available: https://sid.ir/paper/376839/en

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