Analysis of RNA Secondary Structure and qRT-PCR Related to Anti-CD25 CAR Expressed in NK-92 Cell Line

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DEHBASHI MOEIN; HOJATI ZOHREH; MOTOVALI BASHI MAJID; GANJALIKHANI HAKEMI MAZDAK

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Journal: Qom University of Medical Sciences Journal Year:2020 | Volume:14 | Issue:2 Page(s): 1-12

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Title

Analysis of RNA Secondary Structure and qRT-PCR Related to Anti-CD25 CAR Expressed in NK-92 Cell Line

Author(s)

DEHBASHI MOEIN | HOJATI ZOHREH | MOTOVALI BASHI MAJID | GANJALIKHANI HAKEMI MAZDAK | Issue Writer Certificate

Keywords

Computational BiologyQ1

InformaticsQ1

BioinformaticQ1

Real-Time Polymerase Chain ReactionQ1

anti-CD25 CARQ1

TransductionQ1

Tumor Escape-immunologyQ1

Abstract

Background and Objectives: Tumor-infiltrating regulatory T (TI-Treg) cells perform an important function in cancer immune escape. In this study, the third generation CAR construct was designed against human CD25 antigen as the cell surface biomarker of TI-Treg cells. Methods: At first, the construct of anti-CD25 CAR was designed. Using RNAfold web server, the RNA secondary structure was evaluated. Also, NK-92 cell line was transduced using lentiviral vectors. Then, the expression level of anti-CD25 CAR RNA, was assessed by qRT-PCR in NK-92 cells transduced with CAR and mock transfer vectors as well as untreated cells. Results: The RNA secondary structure was stable. Also, the expression level of anti-CD25 CAR RNA in the NK-92 cells transduced by pCDH-513B-1-anti-CD25 CAR transfer vector was significantly higher than NK-92 cells transduced by mock transfer vector and untreated cells (P˂ 0. 0001). Conclusion: The present study on anti-CD25 CAR RNA showed that this type of CAR transcripts were stable and expressed at a high level. In fact, this type of CAR can be further studied in the future as a tool to remove the cancer immune escape in all types of solid and liquid cancers.

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APA: Copy

DEHBASHI, MOEIN, HOJATI, ZOHREH, MOTOVALI BASHI, MAJID, & GANJALIKHANI HAKEMI, MAZDAK. (2020). Analysis of RNA Secondary Structure and qRT-PCR Related to Anti-CD25 CAR Expressed in NK-92 Cell Line. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 14(2 ), 1-12. SID. https://sid.ir/paper/377370/en

Vancouver: Copy

DEHBASHI MOEIN, HOJATI ZOHREH, MOTOVALI BASHI MAJID, GANJALIKHANI HAKEMI MAZDAK. Analysis of RNA Secondary Structure and qRT-PCR Related to Anti-CD25 CAR Expressed in NK-92 Cell Line. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2020;14(2 ):1-12. Available from: https://sid.ir/paper/377370/en

IEEE: Copy

MOEIN DEHBASHI, ZOHREH HOJATI, MAJID MOTOVALI BASHI, and MAZDAK GANJALIKHANI HAKEMI, “Analysis of RNA Secondary Structure and qRT-PCR Related to Anti-CD25 CAR Expressed in NK-92 Cell Line,” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 14, no. 2 , pp. 1–12, 2020, [Online]. Available: https://sid.ir/paper/377370/en

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