ARMS-PCR VERSUS AS-PCR TO EVALUATE JAK2V617F MUTATION IN PATIENTS WITH NON-CML MYELOPROLIFERATIVE NEOPLASMS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NADALI FATEMEH; FERDOWSI SHIRIN; KARIMZADEH PARISA; CHAHARDOULI BAHRAM; EINOLLAHI NAHID; MOUSAVI ASADOLLAH; BAHAR BABAK; DARGAHI HOSEIN; TOOGEH GHOLAMREZA; ALIMOGHADDAM KAMRAN; GHAVAMZADEH ARDESHIR; GHAFFARI SEYED HAMID

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2010 | Volume:68 | Issue:4 Page(s): 207-211

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,336

Download:

Cites:

Information Journal Paper

Title

ARMS-PCR VERSUS AS-PCR TO EVALUATE JAK2V617F MUTATION IN PATIENTS WITH NON-CML MYELOPROLIFERATIVE NEOPLASMS

Author(s)

Keywords

MUTATIONQ3

MYELOPROLIFERATIVEQ3

NEOPLASMQ3

PCRQ3

Abstract

Background: JAK2 is a nonreceptor tyrosine kinase that plays a major role in myeloid disorders. This MUTATION is characterized by a G to T transverse at nucleotide 1849 in exon 12 of the JAK2 gene, located on the chromosome 9p, leading to a substitution of valine to phenylalanine at amino acid position 617 in the JAK2 protein. In this study we compared the amplification refractory MUTATION (ARMS) assay and allele- specific (ASPCR) to evaluate JAK2V617F MUTATION patients with non-CML MYELOPROLIFERATIVE NEOPLASMs (MPNS).Methods: In this experimental study we evaluated JAK2 MUTATION in 58 patients with a known or suspected diagnosis of a MYELOPROLIFERATIVE NEOPLASM by simple randomized sampling. The MUTATION was detected by ARMS-PCR and AS-PCR in patients. In order to verify the methods, amplified products from some patients were sequenced.Results: The JAK2 V617F MUTATION was detected in 86.6% (26/30) of patients with polycythemia vera and 61.5% (8/13) of patients with idiopathic myelofibrosis by ARMSPCR and AS-PCR.46.6% (7.15) of essential thrombocythemia patients were positive using ARMS- PCR method while 53% (8.15) of then were positive when AS- PCR were used. The MUTATION was confirmed by sequencing.Conclusions: The incidence of JAK2 MUTATION using above PCR methods is similar to previous studies. The different results may depend on the molecular technique used.

Cites

No record.

References

Cite

APA: Copy

NADALI, FATEMEH, FERDOWSI, SHIRIN, KARIMZADEH, PARISA, CHAHARDOULI, BAHRAM, EINOLLAHI, NAHID, MOUSAVI, ASADOLLAH, BAHAR, BABAK, DARGAHI, HOSEIN, TOOGEH, GHOLAMREZA, ALIMOGHADDAM, KAMRAN, GHAVAMZADEH, ARDESHIR, & GHAFFARI, SEYED HAMID. (2010). ARMS-PCR VERSUS AS-PCR TO EVALUATE JAK2V617F MUTATION IN PATIENTS WITH NON-CML MYELOPROLIFERATIVE NEOPLASMS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 68(4), 207-211. SID. https://sid.ir/paper/38097/en

Vancouver: Copy

NADALI FATEMEH, FERDOWSI SHIRIN, KARIMZADEH PARISA, CHAHARDOULI BAHRAM, EINOLLAHI NAHID, MOUSAVI ASADOLLAH, BAHAR BABAK, DARGAHI HOSEIN, TOOGEH GHOLAMREZA, ALIMOGHADDAM KAMRAN, GHAVAMZADEH ARDESHIR, GHAFFARI SEYED HAMID. ARMS-PCR VERSUS AS-PCR TO EVALUATE JAK2V617F MUTATION IN PATIENTS WITH NON-CML MYELOPROLIFERATIVE NEOPLASMS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2010;68(4):207-211. Available from: https://sid.ir/paper/38097/en

IEEE: Copy

FATEMEH NADALI, SHIRIN FERDOWSI, PARISA KARIMZADEH, BAHRAM CHAHARDOULI, NAHID EINOLLAHI, ASADOLLAH MOUSAVI, BABAK BAHAR, HOSEIN DARGAHI, GHOLAMREZA TOOGEH, KAMRAN ALIMOGHADDAM, ARDESHIR GHAVAMZADEH, and SEYED HAMID GHAFFARI, “ARMS-PCR VERSUS AS-PCR TO EVALUATE JAK2V617F MUTATION IN PATIENTS WITH NON-CML MYELOPROLIFERATIVE NEOPLASMS,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 68, no. 4, pp. 207–211, 2010, [Online]. Available: https://sid.ir/paper/38097/en

Related Journal Papers