EVALUATION OF JAK2 V617F MUTATION IN IRANIAN PATIENTS WITH NON-CML MYELOPROLIFERATIVE NEOPLASMS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

POUPAK B.; MIRMONGEREH H.; POURFATH ELAH A.A.; SHARIFIAN R.A.; REZVANI HAMID; ELAHI FAZEL; KEYHANI MANOUCHEHR; GHADYANI M.; KAVIANI SAEID; FARAAHANI KOBRA; YOUSEFIAN A.A.F.; JAHANGIRPOUR M.A.; SHAMSIAN M.A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2009 | Volume:5 | Issue:4 (21) Page(s): 237-245

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

3,137

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF JAK2 V617F MUTATION IN IRANIAN PATIENTS WITH NON-CML MYELOPROLIFERATIVE NEOPLASMS

Author(s)

Keywords

JAK2Q4

PROTEIN TYROSINE KINASEQ4

POLYMERASE CHAIN REACTIONQ4

MUTATIONQ3

NEOPLASMSQ2

Abstract

Background and Objectives: Somatic point MUTATION in JAK2 gene is characterized by G to T transversion at nucleotide 1849 in exon 12 in the JAK2 gene and results in autonomous activation of JAK2 protein.JAK2 MUTATION leads to independent cytokine signaling and clonal proliferation of hematopoietic cells in MPNs. Due to absence of any reports for JAK2 V617F MUTATION in Iranian MPNs patients and its important role in diagnosis, we decided to carry out this study.Materials and Methods: In this experimental study, we evaluated JAK2 MUTATION in 100 MPNs patients with diagnosis of PV (n=44), PMF (n=31) and ET (n=25) by simple randomized sampling. After extraction of genomic DNA from whole blood buffy coat, detection of MUTATION was done using allele specific PCR. Agarose gel electrophoresis was used for observation of PCR products. For confirmation of results, PCR-RFLP using BsaXI was applied.Results: Using allele specific PCR and PCR-RFLP, frequency of JAK2 V617F MUTATION was evaluated to be 89%, 61%, and 56% in PV, PMF and ET, respectively.Conclusions: Frequency of JAK2 MUTATION in our study is compatible with previous reports. According to WHO criteria, allele specific PCR can be applied for detection of JAK2 MUTATION in Iranian patients with diagnosis of MPNs.

Cites

No record.

References

No record.

Cite

APA: Copy

POUPAK, B., MIRMONGEREH, H., POURFATH ELAH, A.A., SHARIFIAN, R.A., REZVANI, HAMID, ELAHI, FAZEL, KEYHANI, MANOUCHEHR, GHADYANI, M., KAVIANI, SAEID, FARAAHANI, KOBRA, YOUSEFIAN, A.A.F., JAHANGIRPOUR, M.A., & SHAMSIAN, M.A.. (2009). EVALUATION OF JAK2 V617F MUTATION IN IRANIAN PATIENTS WITH NON-CML MYELOPROLIFERATIVE NEOPLASMS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 5(4 (21)), 237-245. SID. https://sid.ir/paper/78570/en

Vancouver: Copy

POUPAK B., MIRMONGEREH H., POURFATH ELAH A.A., SHARIFIAN R.A., REZVANI HAMID, ELAHI FAZEL, KEYHANI MANOUCHEHR, GHADYANI M., KAVIANI SAEID, FARAAHANI KOBRA, YOUSEFIAN A.A.F., JAHANGIRPOUR M.A., SHAMSIAN M.A.. EVALUATION OF JAK2 V617F MUTATION IN IRANIAN PATIENTS WITH NON-CML MYELOPROLIFERATIVE NEOPLASMS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2009;5(4 (21)):237-245. Available from: https://sid.ir/paper/78570/en

IEEE: Copy

B. POUPAK, H. MIRMONGEREH, A.A. POURFATH ELAH, R.A. SHARIFIAN, HAMID REZVANI, FAZEL ELAHI, MANOUCHEHR KEYHANI, M. GHADYANI, SAEID KAVIANI, KOBRA FARAAHANI, A.A.F. YOUSEFIAN, M.A. JAHANGIRPOUR, and M.A. SHAMSIAN, “EVALUATION OF JAK2 V617F MUTATION IN IRANIAN PATIENTS WITH NON-CML MYELOPROLIFERATIVE NEOPLASMS,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 5, no. 4 (21), pp. 237–245, 2009, [Online]. Available: https://sid.ir/paper/78570/en

Related Journal Papers