IDENTIFICATION OF CONFORMATIONAL EPITOPES ON FRAGMENT CRYSTALLIZABLEREGION OF HUMAN IMMUNOGLOBULIN G BY IMMUNOINFORMATIC

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ROHANI SOHEYLA; HAJIGHASEMI FATEMEH; SEFID FATEMEH

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Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2018 | Volume:76 | Issue:5 Page(s): 321-325

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Information Journal Paper

Title

IDENTIFICATION OF CONFORMATIONAL EPITOPES ON FRAGMENT CRYSTALLIZABLEREGION OF HUMAN IMMUNOGLOBULIN G BY IMMUNOINFORMATIC

Author(s)

ROHANI SOHEYLA | HAJIGHASEMI FATEMEH | SEFID FATEMEH | Issue Writer Certificate

Keywords

EPITOPESQ1

IMMUNOGLOBULIN GQ1

IMMUNOLOGYQ2

Abstract

Background: Immunoglobulins are a group of proteins have important role in defenseagainst microorganisms. Human immunoglobulins are divided into five classes: IgA, IgM, IgD, IgE and IgG. IMMUNOGLOBULIN G (IgG) is the highest abundant antibody inserum and extravascular fluids. The extent of serum IgG is related to severity of severaldiseases such as infections, so IgG has great diagnostic worth. Accurate measurementof IgG, needs exact and sensitive diagnostic instruments such as human IgG-specificmonoclonal antibodies. Moreover, targeting of IgG has been useful in treatment of anumber of diseases. According to experimental studies the Fc region of human IgG ishighly immunogenic. Immunoinformatic is a division of IMMUNOLOGY uses the computationalbiology for more precise diagnosis of diseases. The aim of this study was determinationof conformational EPITOPES in the fragment of crystallizable (Fc) fragmentof human IgG by immunoinformatic. Methods: The amino acid residues and third structure of reference human IgG werefound in protein data bank (PDB). Second IgG structure was defined by Phyre2 software(http: //www. sbg. bio. ic. ac. uk/~phyre2/). Conformational EPITOPES of the Fc fragmentin human IgG were specified by ElliPro (http: //tools. iedb. org/ellipro/) and Disco-Tope (http: //www. cbs. dtu. dk/services/DiscoTope) softwares. Results: In this study two conformational EPITOPES (one in constant heavy chain 2(CH2) domain and another one common between CH2 and CH3 domains) sited in Fcfragment of human IgG were determined by ElliPro software. Also, two conformationalEPITOPES (Both common between CH2 and CH3 domains) located to Fc fragment ofhuman IgG were determined by DiscoTope software. Conclusion: In this study a number of conformational EPITOPES located to Fc fragmentof human IgG were determined by two immunoinformatic softwares (ElliPro and DiscoTope). The EPITOPES recognized by both softwares were situated in CH2, CH3 or bothof these domains in the human IgG heavy chain. Thus, it seems that CH2 and CH3 domainsof Fc region in human IgG are highly immunogenic. Moreover, ElliPro and DiscoTopesoftwares can be useful tools for identification of EPITOPES located to Fc fragmentof human IgG.

Cites

In Silico Prediction of Continuous Epitopes on Human immunoglobulin Light Chains

References

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APA: Copy

ROHANI, SOHEYLA, HAJIGHASEMI, FATEMEH, & SEFID, FATEMEH. (2018). IDENTIFICATION OF CONFORMATIONAL EPITOPES ON FRAGMENT CRYSTALLIZABLEREGION OF HUMAN IMMUNOGLOBULIN G BY IMMUNOINFORMATIC. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 76(5 ), 321-325. SID. https://sid.ir/paper/38213/en

Vancouver: Copy

ROHANI SOHEYLA, HAJIGHASEMI FATEMEH, SEFID FATEMEH. IDENTIFICATION OF CONFORMATIONAL EPITOPES ON FRAGMENT CRYSTALLIZABLEREGION OF HUMAN IMMUNOGLOBULIN G BY IMMUNOINFORMATIC. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2018;76(5 ):321-325. Available from: https://sid.ir/paper/38213/en

IEEE: Copy

SOHEYLA ROHANI, FATEMEH HAJIGHASEMI, and FATEMEH SEFID, “IDENTIFICATION OF CONFORMATIONAL EPITOPES ON FRAGMENT CRYSTALLIZABLEREGION OF HUMAN IMMUNOGLOBULIN G BY IMMUNOINFORMATIC,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 76, no. 5 , pp. 321–325, 2018, [Online]. Available: https://sid.ir/paper/38213/en

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