Molecular evaluation of candida species as etiologic agents of onychomycosis

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LOTFALI ENSIEH; MIRAMIN MOHAMMADI AKRAM; Shahrzad Mahshid; KHAMESIPOUR ALI; FIROOZ ALIREZA; Fattahi Azam

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Journal: Dermatology and Cosmetics Year:2020 | Volume:11 | Issue:1 Page(s): 28-34

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Title

Molecular evaluation of candida species as etiologic agents of onychomycosis

Author(s)

Keywords

onychomycosisQ1

yeastsQ1

candida albicansQ1

candida parapsilosisQ1

molecular diagnosisQ3

PCR-RFLPQ2

Abstract

Background and Aim: The aim of the present study is rapid and precise identification of yeast species isolated from nail of patients with clinical features of onychomycosis using PCR-RFLP technique. Methods: 50 patients with involvement of 29 fingernails and 21 toenails were enrolled in the study. Different yeast species were identified by conventional mycological. The PCR-RFLP was tested on yeast isolated and the PCR-RFLP products were separated by electrophoresis in 2% agarose gel, with DNA stain. Results: The main causative agents were yeasts in 12 cases (24%). candida albicans was the most commonly isolated yeast species followed by candida parapsilosis (6%), and Candida glabrata (4 %). Conclusion: PCR-RFLP method using ITS1-ITS4 primers and MspI restriction enzymes is a rapid, accurate and cost-effective method for specific diagnosis of the most prevalent candida spp. Its ability to detect low amounts of fungal DNA in patient samples in 6-8 hours could be useful for clinical laboratories for optimal management of these infections.

Cites

Antifungal susceptibility to azoles drugs in Candida species isolated from patients with onychomycosis

References

Determination of antimicotic susceptibility pattern of Candida species isolated from patients with symptomatic candiduria

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APA: Copy

LOTFALI, ENSIEH, MIRAMIN MOHAMMADI, AKRAM, Shahrzad, Mahshid, KHAMESIPOUR, ALI, FIROOZ, ALIREZA, & Fattahi, Azam. (2020). Molecular evaluation of candida species as etiologic agents of onychomycosis. DERMATOLOGY AND COSMETIC, 11(1 ), 28-34. SID. https://sid.ir/paper/382572/en

Vancouver: Copy

LOTFALI ENSIEH, MIRAMIN MOHAMMADI AKRAM, Shahrzad Mahshid, KHAMESIPOUR ALI, FIROOZ ALIREZA, Fattahi Azam. Molecular evaluation of candida species as etiologic agents of onychomycosis. DERMATOLOGY AND COSMETIC[Internet]. 2020;11(1 ):28-34. Available from: https://sid.ir/paper/382572/en

IEEE: Copy

ENSIEH LOTFALI, AKRAM MIRAMIN MOHAMMADI, Mahshid Shahrzad, ALI KHAMESIPOUR, ALIREZA FIROOZ, and Azam Fattahi, “Molecular evaluation of candida species as etiologic agents of onychomycosis,” DERMATOLOGY AND COSMETIC, vol. 11, no. 1 , pp. 28–34, 2020, [Online]. Available: https://sid.ir/paper/382572/en

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