PRIMARY CULTURE OF HUMAN SKIN MELANOCYTE AND COMPARISON OF CULTURE IN THE PRESENCE AND ABSENCE OF PHORBOL ESTER

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YARANI REZA; MANSOURI KAMRAN; BIDMESHKIPOUR ALI; MEHRABI MARYAM; EBRAHIMI ALI; GHOLAMI KEYKAVOS; YARI KHEIROLLAH; MOSTAFAIE ALI

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Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2013 | Volume:71 | Issue:3 Page(s): 139-148

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Title

PRIMARY CULTURE OF HUMAN SKIN MELANOCYTE AND COMPARISON OF CULTURE IN THE PRESENCE AND ABSENCE OF PHORBOL ESTER

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Keywords

EPIDERMISQ3

GROWTH FACTORQ3

MELANOCYTESQ2

PHORBOL ESTERQ2

SKINQ2

Abstract

Background: Primary culture takes place following the cell isolation from tissues. Isolation and culture of MELANOCYTES based on their roll in the protection of body against hazardous sun rays, production of SKIN, cornea and hair color is really important. This study was done to set isolation, culture and proliferation of MELANOCYTES from children foreskin and adult eyelashes, and also comparison of two types of melanocyte culture medium.Methods: Human foreskin and eyelash samples were used for melanocyte isolation and culture. After isolation of EPIDERMIS from dermis, EPIDERMIS cell suspensions were prepared by enzymatic digestion. The isolated cells were cultured in two melanocyte selective culture media. Immunocytochemistary and reverse transcription-polymerase chain reaction (RT-PCR) assays were used for confirmation of isolated and cultured MELANOCYTES.Results: Our results indicated that isolated melanocyte cultured in the selective medium without PHORBOL ESTERs is better than the MELANOCYTES cultured in selective medium containing PHORBOL ESTERs not only morphologically but also physiologically and from the aspect of cell adhesion. In addition, the results showed that isolated melanocyte from adult eyelashes are more dendritic than MELANOCYTES isolated from children foreskin.Conversely, our results indicated that the number of cell passages in melanocyte isolated from foreskin is more than MELANOCYTES isolated from adult eyelashes.Conclusion: MELANOCYTES cultured in selective medium containing convenient GROWTH FACTORs in absence of PHORBOL ESTERs show more native physiological and adhesive properties. In addition, melanocyte isolated from younger tissues such as foreskin have better proliferative and sub-culturing properties so we suggest isolation and culture of younger tissues.

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APA: Copy

YARANI, REZA, MANSOURI, KAMRAN, BIDMESHKIPOUR, ALI, MEHRABI, MARYAM, EBRAHIMI, ALI, GHOLAMI, KEYKAVOS, YARI, KHEIROLLAH, & MOSTAFAIE, ALI. (2013). PRIMARY CULTURE OF HUMAN SKIN MELANOCYTE AND COMPARISON OF CULTURE IN THE PRESENCE AND ABSENCE OF PHORBOL ESTER. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 71(3), 139-148. SID. https://sid.ir/paper/38341/en

Vancouver: Copy

YARANI REZA, MANSOURI KAMRAN, BIDMESHKIPOUR ALI, MEHRABI MARYAM, EBRAHIMI ALI, GHOLAMI KEYKAVOS, YARI KHEIROLLAH, MOSTAFAIE ALI. PRIMARY CULTURE OF HUMAN SKIN MELANOCYTE AND COMPARISON OF CULTURE IN THE PRESENCE AND ABSENCE OF PHORBOL ESTER. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2013;71(3):139-148. Available from: https://sid.ir/paper/38341/en

IEEE: Copy

REZA YARANI, KAMRAN MANSOURI, ALI BIDMESHKIPOUR, MARYAM MEHRABI, ALI EBRAHIMI, KEYKAVOS GHOLAMI, KHEIROLLAH YARI, and ALI MOSTAFAIE, “PRIMARY CULTURE OF HUMAN SKIN MELANOCYTE AND COMPARISON OF CULTURE IN THE PRESENCE AND ABSENCE OF PHORBOL ESTER,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 71, no. 3, pp. 139–148, 2013, [Online]. Available: https://sid.ir/paper/38341/en

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