DIFFERENTIATION OF ADIPOSE DERIVED STEM CELLS TO NEURON LIKE CELLS ON ALIGNED NANOFIBROUS SCAFFOLD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ESFANDIARI B.; SOLIEMANI M.; KAVIANI S.; PARIVAR K.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Advances in Medical and Biomedical Research Year:2017 | Volume:25 | Issue:108 Page(s): 81-95

Download Full-Text

Persian Verion

View:

872

Download:

Cites:

Information Journal Paper

Title

DIFFERENTIATION OF ADIPOSE DERIVED STEM CELLS TO NEURON LIKE CELLS ON ALIGNED NANOFIBROUS SCAFFOLD

Author(s)

ESFANDIARI B. | SOLIEMANI M. | KAVIANI S. | PARIVAR K. | Issue Writer Certificate

Keywords

NEURAL TISSUE ENGINEERINGQ1

ADIPOSE DERIVED STEM CELLSQ2

NEURON LIKE CELLQ1

ALIGNED PCL NANOFIBER SCAFFOLDQ1

DIFFERENTIATIONQ2

Abstract

Background and Objective: DIFFERENTIATION of mesenchymal stem cells into neurons has great potential in the therapy of damaged nerve tissue. NEURAL TISSUE ENGINEERING offers tremendous promise to combat the effects of disease, aging and injury in the nervous system. The aim of this study was to investigate the DIFFERENTIATION of ADIPOSE DERIVED STEM CELLS to NEURON LIKE CELLs on aligned nanofibrous scaffold.Materials and Methods: In this study, the surface of aligned polycaprolactone (PCL) nanofibrous scaffolds were modified with O2 plasma treatment to enhance their hydrophilicity in order to differentiate hADSCs into NEURON LIKE CELLs. The chemical and mechanical characteristics of electrospined aligned PCL fibers were determined using scanning electron microscope (SEM) and the calculation of contact angles. The DIFFERENTIATION of ADIPOSE DERIVED STEM CELLS was performed using neuronal inducing factors including bFGF, forskolin and NGF with 1% FBS for 8 days.Results: Real-time PCR analysis indicated the upregulation of Map2, NSE, and NFM genes and downregulation of Nestin. Also the expression of neuronal proteins such as MAP2 andâTubullin were confirmed by immunocytochemistry. It was found that the direction of cell elongation and neurite outgrowth on the aligned nanofibrous scaffolds is parallel to the direction of fibers.Conclusion: The results of the present study proposed that p-PCL is a cost-effective material for DIFFERENTIATION of hADSCs into NEURON LIKE CELLs and could apply in nerve tissue repair.

Cites

No record.

References

Cite

APA: Copy

ESFANDIARI, B., SOLIEMANI, M., KAVIANI, S., & PARIVAR, K.. (2017). DIFFERENTIATION OF ADIPOSE DERIVED STEM CELLS TO NEURON LIKE CELLS ON ALIGNED NANOFIBROUS SCAFFOLD. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, 25(108), 81-95. SID. https://sid.ir/paper/61237/en

Vancouver: Copy

ESFANDIARI B., SOLIEMANI M., KAVIANI S., PARIVAR K.. DIFFERENTIATION OF ADIPOSE DERIVED STEM CELLS TO NEURON LIKE CELLS ON ALIGNED NANOFIBROUS SCAFFOLD. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH[Internet]. 2017;25(108):81-95. Available from: https://sid.ir/paper/61237/en

IEEE: Copy

B. ESFANDIARI, M. SOLIEMANI, S. KAVIANI, and K. PARIVAR, “DIFFERENTIATION OF ADIPOSE DERIVED STEM CELLS TO NEURON LIKE CELLS ON ALIGNED NANOFIBROUS SCAFFOLD,” JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, vol. 25, no. 108, pp. 81–95, 2017, [Online]. Available: https://sid.ir/paper/61237/en

Related Journal Papers