FREQUENCY OF CHLAMYDIA TRACHOMATIS IN GENITAL SPECIMENS: KERMAN CITY, PCR METHOD

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KHALILI MOHAMMAD; ATAPOUR M.; AALEI B.SH.; AZIZ ELAHI GH.A.; AZIZ ELAHI S.

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Journal: Tehran University Medical Journal Year:2008 | Volume:66 | Issue:2 Page(s): 127-130

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Title

FREQUENCY OF CHLAMYDIA TRACHOMATIS IN GENITAL SPECIMENS: KERMAN CITY, PCR METHOD

Author(s)

Keywords

CHLAMYDIA TRACHOMATISQ3

PCRQ3

UERTHRITISQ3

CERVICITISQ3

KERMANQ3

Abstract

Background: CHLAMYDIA TRACHOMATIS (CT) is an obligate intracellular bacterium that causes genital disease and the most common sexually transmitted infection in the world. The most frequent risk factors associated with chlamydial infection are related to sexual behavior, multiple partners, and inconsistent condom use. Presenting primarily as urtheritis in men and CERVICITIS in women, CT a major cause of chronic pelvic inflammatory disease and subsequent infertility in women, eye and lung infection in newborns and other manifestations. Identification of CT-infected patients may prevent its spread and thereby reduce the high morbidity associated with CT infections. Polymerase chain reaction (PCR) is a sensitive and specific method for the detection of small quantity of bacterial DNA in clinical samples. The aim of this study was to determine the frequency of C. trachomatis by PCR in genital samples from patients in the city of KERMAN.Methods: A total of 130 genital samples including 64 endocervical and 66 urethral swab samples were collected by physicians. Nucleic acid was extracted from each sample using a commercial DNA extraction kit. PCR primers specific for a conserved region of the C. trachomatis omp2 gene, encoding an outer membrane protein, were used for amplification.Results: A total of 9.2% (6.25% of CERVICITIS and 12.1% of urethritis) of the samples were found positive for CT using this PCR method.Conclusions: The present study shows a high prevalence of CT infection, especially in men with urethritis. Such patients should be referred to genitourinary clinics for treatment and partner notification. Given its worldwide prevalence, further CT studies on more populations are needed to assess potential public health implications of these infections.

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References

DETECTION OF CHLAMYDIA TRACHOMATIS AND NEISSERIA GONORRHOAE BY PCR AND MULTIPLEX PCR FROM NON-INVASIVE GENITO-URINARY SPECIMEN OF PATIENTS WITH URETHRITIS

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APA: Copy

KHALILI, MOHAMMAD, ATAPOUR, M., AALEI, B.SH., AZIZ ELAHI, GH.A., & AZIZ ELAHI, S.. (2008). FREQUENCY OF CHLAMYDIA TRACHOMATIS IN GENITAL SPECIMENS: KERMAN CITY, PCR METHOD. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 66(2), 127-130. SID. https://sid.ir/paper/38864/en

Vancouver: Copy

KHALILI MOHAMMAD, ATAPOUR M., AALEI B.SH., AZIZ ELAHI GH.A., AZIZ ELAHI S.. FREQUENCY OF CHLAMYDIA TRACHOMATIS IN GENITAL SPECIMENS: KERMAN CITY, PCR METHOD. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2008;66(2):127-130. Available from: https://sid.ir/paper/38864/en

IEEE: Copy

MOHAMMAD KHALILI, M. ATAPOUR, B.SH. AALEI, GH.A. AZIZ ELAHI, and S. AZIZ ELAHI, “FREQUENCY OF CHLAMYDIA TRACHOMATIS IN GENITAL SPECIMENS: KERMAN CITY, PCR METHOD,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 66, no. 2, pp. 127–130, 2008, [Online]. Available: https://sid.ir/paper/38864/en

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