Accuracy of Indirect ELISA Prepared from Recombinant Bp26 Gene of Brucella melitensis in Diagnosis of Human Brucellosis

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HOSEINI S.D.; GHAZNAVIRAD E.; FARAZI A.A.

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Journal: IRANIAN SOUTH MEDICAL JOURNAL (ISMJ) Year:2020 | Volume:23 | Issue:4 Page(s): 292-301

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Title

Accuracy of Indirect ELISA Prepared from Recombinant Bp26 Gene of Brucella melitensis in Diagnosis of Human Brucellosis

Author(s)

HOSEINI S.D. | GHAZNAVIRAD E. | FARAZI A.A. | Issue Writer Certificate

Keywords

BrucellosisQ2

Recombinant proteinsQ2

ELISAQ2

bp26 geneQ1

Abstract

Background: Considering the prevalence of Brucellosis in Iran, it is necessary to choose a specific and sensitive laboratory method to diagnose it in a rapid and timely manner. The aim of this study was to assess the accuracy of indirect enzyme-linked immunosorbent assay (ELISA) for detecting Brucellosis in humans in order to have an appropriate alternative to conventional tests such as Wright, 2ME, and commercial ELISA kits. Materials and Methods: In this study, the recombinant protein produced from the gene (omp28) bp26 Brucella melitensis was used as an antigen for coating microplate wells. A total of 124 serum samples of normal healthy individuals (n=62) and patients with acute Brucellosis (n=62) approved by STA and 2 ME tests were entered into the study. The data were analyzed in SPSS (ver. 18). Results: The mean age was 39. 8± 13. 5 years in the patient group and 36. 1± 12. 7 years in the healthy group. Furthermore, 66. 1% of the patients were male and 62. 9% lived in rural regions, while these figures were respectively 71% and 45. 2% in the healthy group. The sensitivity of 92% and specificity of 87% and a positive predictive value of 88% and a negative predictive value of 92% and an accuracy of 90% were determined for ELISA kit used in this study. Conclusion: The ELISA diagnostic kit reacted to most of the positive human sera. However, this kit needs to be further evaluated with a larger sample size of clinical specimens from different regions and with various clinical forms of human Brucellosis.

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APA: Copy

HOSEINI, S.D., GHAZNAVIRAD, E., & FARAZI, A.A.. (2020). Accuracy of Indirect ELISA Prepared from Recombinant Bp26 Gene of Brucella melitensis in Diagnosis of Human Brucellosis. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), 23(4 ), 292-301. SID. https://sid.ir/paper/391708/en

Vancouver: Copy

HOSEINI S.D., GHAZNAVIRAD E., FARAZI A.A.. Accuracy of Indirect ELISA Prepared from Recombinant Bp26 Gene of Brucella melitensis in Diagnosis of Human Brucellosis. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)[Internet]. 2020;23(4 ):292-301. Available from: https://sid.ir/paper/391708/en

IEEE: Copy

S.D. HOSEINI, E. GHAZNAVIRAD, and A.A. FARAZI, “Accuracy of Indirect ELISA Prepared from Recombinant Bp26 Gene of Brucella melitensis in Diagnosis of Human Brucellosis,” IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), vol. 23, no. 4 , pp. 292–301, 2020, [Online]. Available: https://sid.ir/paper/391708/en

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