DESIGN AND CONSTRUCTION OF EXPRESSION VECTOR CONTAINING THE CHIMERIC ANTIGEN OF B. MELITENSIS OMP25-OMP31 TO PRODUCTION OF A VACCINE AGAINST BRUCELLOSIS

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KHABIRI ALI AKBAR; SOLEIMANI MOHAMMAD; SEKHAVATI MOHAMMAD HADI; TAHMOORESPUR MOJTABA

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Journal: MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:40 | Issue:5 Page(s): 32-37

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Title

DESIGN AND CONSTRUCTION OF EXPRESSION VECTOR CONTAINING THE CHIMERIC ANTIGEN OF B. MELITENSIS OMP25-OMP31 TO PRODUCTION OF A VACCINE AGAINST BRUCELLOSIS

Author(s)

KHABIRI ALI AKBAR | SOLEIMANI MOHAMMAD | SEKHAVATI MOHAMMAD HADI | TAHMOORESPUR MOJTABA | Issue Writer Certificate

Keywords

BRUCELLA MELITENSISQ3

VECTORQ1

OMPSQ1

LINKERQ1

CHIMERAQ1

Abstract

Background: Brucella is gram-negative intracellular bacterial pathogens of both humans and animals. Among the sixrecognized Brucella species, BRUCELLA MELITENSIS is the main etiologic agent involved in ovine and caprine brucellosis and is alsothe most pathogenic species of humans. Methods: We amplified by specific primers, two of the best outer membrane antigens Omp31 and Omp25 for this disease, and the construction of recombinant chimeric Omp31-Omp25 combination of them, with using the (EAAAK)2 LINKER. Results: The sequence analysis results showed that strain Rev1 of melitensis for Omp25 antigen has most homology with thestrains melitensis M16 and also Omp31 antigen showed the most homology with of B. Ovis melitensis species. Conclusion: Then recombinant CHIMERA Omp31-Omp25 was cloned into Pet32a VECTOR and transform into BL21 (DE3) hostcell for expression.

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APA: Copy

KHABIRI, ALI AKBAR, SOLEIMANI, MOHAMMAD, SEKHAVATI, MOHAMMAD HADI, & TAHMOORESPUR, MOJTABA. (2019). DESIGN AND CONSTRUCTION OF EXPRESSION VECTOR CONTAINING THE CHIMERIC ANTIGEN OF B. MELITENSIS OMP25-OMP31 TO PRODUCTION OF A VACCINE AGAINST BRUCELLOSIS. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 40(5 ), 32-37. SID. https://sid.ir/paper/46524/en

Vancouver: Copy

KHABIRI ALI AKBAR, SOLEIMANI MOHAMMAD, SEKHAVATI MOHAMMAD HADI, TAHMOORESPUR MOJTABA. DESIGN AND CONSTRUCTION OF EXPRESSION VECTOR CONTAINING THE CHIMERIC ANTIGEN OF B. MELITENSIS OMP25-OMP31 TO PRODUCTION OF A VACCINE AGAINST BRUCELLOSIS. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;40(5 ):32-37. Available from: https://sid.ir/paper/46524/en

IEEE: Copy

ALI AKBAR KHABIRI, MOHAMMAD SOLEIMANI, MOHAMMAD HADI SEKHAVATI, and MOJTABA TAHMOORESPUR, “DESIGN AND CONSTRUCTION OF EXPRESSION VECTOR CONTAINING THE CHIMERIC ANTIGEN OF B. MELITENSIS OMP25-OMP31 TO PRODUCTION OF A VACCINE AGAINST BRUCELLOSIS,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 40, no. 5 , pp. 32–37, 2019, [Online]. Available: https://sid.ir/paper/46524/en

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