Genetic Diversity and Phylogenetic Relationship of Clinical Isolates of Brucella melitensis Based on Gene Polymorphism of β Subunit of RNA Polymerase (rpoB) Gene in Iran

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Bazrgari Nasim; GAROOSI GHASEM ALI; DADAR MARYAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2020 | Volume:14 | Issue:5 Page(s): 425-440

Download Full-Text

Persian Verion

View:

237

Download:

220

Cites:

Information Journal Paper

Title

Genetic Diversity and Phylogenetic Relationship of Clinical Isolates of Brucella melitensis Based on Gene Polymorphism of β Subunit of RNA Polymerase (rpoB) Gene in Iran

Author(s)

Bazrgari Nasim | GAROOSI GHASEM ALI | DADAR MARYAM | Issue Writer Certificate

Keywords

RpoBQ1

Brucella melitensisQ1

SNP analysisQ1

PhylogeneticQ1

Abstract

Background: The prevalence of Brucella infections in animals and humans has indicated the important need for different regional/local reference laboratories to use valid species-determining approaches to facilitate and compare data exchange. The purpose of current study was to evaluate the RNA Polymerase Beta Subunit (RpoB) as a molecular marker in Brucella species differentiation and to determine the genotype of Brucella melitensis species using single-nucleotide polymorphism (SNP) analysis. Materials & Methods: In this study, blood and cerebrospinal fluid (CSF) samples were taken from 108 patients with brucellosis. After culturing the samples insupplemented Brucella agar, eleven isolates of Brucella bacteria were isolated and identified by classical and molecular biotyping methods. Then the complete sequence of their RpoB gene was multiplied and sequenced. Sequencing results were analyzed by Mega6 program. Results: According to the results, the RpoB gene was able to differentiate between Brucella species and other bacteria. Moreover, the RpoB typing grouped the majority of Iranian isolates in the RpoB type 2, while only one strain belonged to the RpoB type 1. Among the 10 isolates of RpoB type 2, there are six different isolates with only one unique type-2 SNPs in codon 985, which gives rise to new genotype 2 variants. Conclusion: Our results shown a high discriminative power of RpoB gene among B. melitensis strains from some regions of Iran, which leads to accurate genotype and identification of these bacteria.

Cites

No record.

References

Cite

APA: Copy

Bazrgari, Nasim, GAROOSI, GHASEM ALI, & DADAR, MARYAM. (2020). Genetic Diversity and Phylogenetic Relationship of Clinical Isolates of Brucella melitensis Based on Gene Polymorphism of β Subunit of RNA Polymerase (rpoB) Gene in Iran. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 14(5 ), 425-440. SID. https://sid.ir/paper/391762/en

Vancouver: Copy

Bazrgari Nasim, GAROOSI GHASEM ALI, DADAR MARYAM. Genetic Diversity and Phylogenetic Relationship of Clinical Isolates of Brucella melitensis Based on Gene Polymorphism of β Subunit of RNA Polymerase (rpoB) Gene in Iran. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2020;14(5 ):425-440. Available from: https://sid.ir/paper/391762/en

IEEE: Copy

Nasim Bazrgari, GHASEM ALI GAROOSI, and MARYAM DADAR, “Genetic Diversity and Phylogenetic Relationship of Clinical Isolates of Brucella melitensis Based on Gene Polymorphism of β Subunit of RNA Polymerase (rpoB) Gene in Iran,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 14, no. 5 , pp. 425–440, 2020, [Online]. Available: https://sid.ir/paper/391762/en

Related Journal Papers