ANTIBACTERIAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON BRUCELLA MELITENSIS M16 AND BRUCELLA ABORTUS S99 IN VITRO AND IN VIVO

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ABDOLAHZADE P.; SHAPOURI R.; NASIRI SEMNANI SH.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS) Year:2011 | Volume:11 | Issue:3 (41) Page(s): 218-227

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,226

Download:

Cites:

Information Journal Paper

Title

ANTIBACTERIAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON BRUCELLA MELITENSIS M16 AND BRUCELLA ABORTUS S99 IN VITRO AND IN VIVO

Author(s)

ABDOLAHZADE P. | SHAPOURI R. | NASIRI SEMNANI SH. | Issue Writer Certificate

Keywords

BRUCELLA MELITENSIS M16Q2

BRUCELLA ABORTUS S99Q2

EUCALYPTUS GLOBULUS EXTRACTSQ2

ANTIMICROBIAL EFFECTQ3

Abstract

Background & Objectives: Brucellosis is an infectious disease caused by intracellular pathogens of the genus Brucella that have their natural reservoir in domestic and wild animals. Many studies show that herbal medicines have been used safely and successfully to treat bacterial diseases without significant side effects and drug resistance problems.Methods: In this study aquatic, alcoholic and acetonic extracts of Eucalyptus globulus leaves were prepared, then MIC and MBC of extracts for B. melitensis 16M and B. abortus S99 were determined by broth macrodilution and agar well diffusion methods. In animal model study, 5×05 CFU/mL of Brucellae was injected intraperitoneally (i.p) to female BALB/c mice. After 24 hours, 0.5mL (equivalent MBC) of each EUCALYPTUS GLOBULUS EXTRACTS was injected (i.p) After 7 days, in spleen the colonies of brucellae were counted on Muller-Hinton agar as standard protocol.Results: The MIC and MBC of Eucalyptus globulus for B. melitensis M16 and B. abortus S99 were 1:80 (10.81 mg/mL) and 1:40 (21.62 mg/mL) for aquatic extract, 1:1280 (0.64 mg/mL) and 1:640 (1.29 mg/mL) for acetonic extract, and 1:2560 (0.31 mg/mL) and 1:1280 (0.63 mg/mL), for ethanolic extract respectively. In culture of spleen supernatant (in vivo), after 48 hours, the average grown B. melitensis 16M colonies for aquatic, ethanolic and acetonic extracts were 5×103 CFU/ml, 2×102 CFU/ml and 6×102 CFU/ml, respectively in comparison with control group (4×1010 CFU/ml). These results for B.abortus S99 were 3×103 CFU/ml, 1×102CFU/ml and 3×102 CFU/ml, respectively in comparison with control group (9×109 CFU/ml) The results showed that bacterial load was significantly decreased in all experimental groups (p<0.01).Conclusion: The results of in vitro and in vivo indicate that ethanolic and acetonic extracts of Eucalyptus globulus have more effective antimicrobial activity on B.melitensis M16 and B.abortus S99 than aquatic extract. It seems that the extracts Eucalyptus globulus can be used in treatment of human and animal brucellosis.

Cites

EVALUATION OF THE ANTIBACTERIAL EFFECTS OF ARCTIUM LAPPA EXTRACTS ON BRUCELLA MELITENSIS 16M IN THE ANIMAL MODEL AND MACROPHAGE CULTURE

References

No record.

Cite

APA: Copy

ABDOLAHZADE, P., SHAPOURI, R., & NASIRI SEMNANI, SH.. (2011). ANTIBACTERIAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON BRUCELLA MELITENSIS M16 AND BRUCELLA ABORTUS S99 IN VITRO AND IN VIVO. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 11(3 (41)), 218-227. SID. https://sid.ir/paper/59673/en

Vancouver: Copy

ABDOLAHZADE P., SHAPOURI R., NASIRI SEMNANI SH.. ANTIBACTERIAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON BRUCELLA MELITENSIS M16 AND BRUCELLA ABORTUS S99 IN VITRO AND IN VIVO. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2011;11(3 (41)):218-227. Available from: https://sid.ir/paper/59673/en

IEEE: Copy

P. ABDOLAHZADE, R. SHAPOURI, and SH. NASIRI SEMNANI, “ANTIBACTERIAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON BRUCELLA MELITENSIS M16 AND BRUCELLA ABORTUS S99 IN VITRO AND IN VIVO,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 11, no. 3 (41), pp. 218–227, 2011, [Online]. Available: https://sid.ir/paper/59673/en

Related Journal Papers