INHIBITION OF BREAST CANCER METASTASIS BY USING MIR-31-MIMIC IN CANCER STEM CELL RICH MDA-MB231 CELL LINE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FAROKHIMANESH SAMILA; FOROUZANDEH MOGHADAM MAHDI; EBRAHIMI MARZIEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Tehran University Medical Journal Year:2015 | Volume:73 | Issue:1 Page(s): 1-10

Download Full-Text

Persian Verion

View:

795

Download:

Cites:

Information Journal Paper

Title

INHIBITION OF BREAST CANCER METASTASIS BY USING MIR-31-MIMIC IN CANCER STEM CELL RICH MDA-MB231 CELL LINE

Author(s)

FAROKHIMANESH SAMILA | FOROUZANDEH MOGHADAM MAHDI | EBRAHIMI MARZIEH | Issue Writer Certificate

Keywords

BREAST NEOPLASMSQ2

MIR-31-MIMICQ2

NEOPLASM METASTASISQ2

Abstract

Background: Metastasis associated miRNA (metastamiR) opened a new field of antimetastatic therapy which have a great potential of treatment for the most lethal aspect of cancer, metastasis. The pleiotropic nature of gene regulation exhibited by certain miRNAs that showed that miRNAs might be endowed with a capacity to function as crucial modulators of tumor metastasis. MiR-31 is a pleiotropic anti-metastatic miRNA whose expression decreased significantly in metastatic breast cancer cells. MiR-31 has multiple roles in metastasis cascade. Therefore, using the miR-31-restoration based therapy could be an efficient anti-metastatic strategy for cancer therapy.Methods: This research was performed from May 2014 to October 2015 in Tarbiat Modares University in Tehran, Iran. The double-strand oligo of mature miR-31 was cloned into pcDNA 6.2gw/EmGFP according to the manufacturer instruction. The MDA-MB231, MCF-7 breast cancer cell lines were cultured and their miRNAs have been extracted. The expression of miR-31 has been quantified by Real time-PCR before transfection of construct contained miR-31 into two cell lines and in normal breast cells. Then the constructs contain miR-31 have been transfected in to two cell lines. The expression of miR-31 has been quantified after 48 hours. Scratch and invasion assay have been carried out for assessing the level of migration and invasion.Results: The result of Real time-PCR before transfection of constructs contained miR- 31 have been shown 4 fold and more than 100 fold reduction in expression of miR-31 in MCF-7 and MDA-MB231 respectively in comparison to miR-31 expression in normal breast cells, but after transfection of miR-31 construct to MDA-MB231 the quantification of expression showed the significant increase in mir-31 expression and 20 fold reduction in invasive and 10 fold reduction in migratory characteristics of MDAMB231 in comparison to MCF-7.Conclusion: Metastasis associated miRNA have been represented a promising candidates in the field of anti-metastatic therapy and miR-31 as a powerful member of this family can function very effectively in order to inhibit the metastasis and introduce the new possibility of metastasis inhibition.

Cites

No record.

References

Cite

APA: Copy

FAROKHIMANESH, SAMILA, FOROUZANDEH MOGHADAM, MAHDI, & EBRAHIMI, MARZIEH. (2015). INHIBITION OF BREAST CANCER METASTASIS BY USING MIR-31-MIMIC IN CANCER STEM CELL RICH MDA-MB231 CELL LINE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 73(1), 1-10. SID. https://sid.ir/paper/39244/en

Vancouver: Copy

FAROKHIMANESH SAMILA, FOROUZANDEH MOGHADAM MAHDI, EBRAHIMI MARZIEH. INHIBITION OF BREAST CANCER METASTASIS BY USING MIR-31-MIMIC IN CANCER STEM CELL RICH MDA-MB231 CELL LINE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2015;73(1):1-10. Available from: https://sid.ir/paper/39244/en

IEEE: Copy

SAMILA FAROKHIMANESH, MAHDI FOROUZANDEH MOGHADAM, and MARZIEH EBRAHIMI, “INHIBITION OF BREAST CANCER METASTASIS BY USING MIR-31-MIMIC IN CANCER STEM CELL RICH MDA-MB231 CELL LINE,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 73, no. 1, pp. 1–10, 2015, [Online]. Available: https://sid.ir/paper/39244/en

Related Journal Papers