INVESTIGATION OF TELOMERASE ACTIVITY AND HTERT GENE EXPRESSION IN MCF7 CELLS TREATED WITH PAPAVERINE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KAZEMI NOUREINI S.; NABIUNI M.; NOORI Z.A.; KHOSROJERDI F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Sabzevar University of Medical Sciences Year:2013 | Volume:20 | Issue:2 (68) Page(s): 121-132

Download Full-Text

Persian Verion

View:

1,814

Download:

Cites:

Information Journal Paper

Title

INVESTIGATION OF TELOMERASE ACTIVITY AND HTERT GENE EXPRESSION IN MCF7 CELLS TREATED WITH PAPAVERINE

Author(s)

KAZEMI NOUREINI S. | NABIUNI M. | NOORI Z.A. | KHOSROJERDI F. | Issue Writer Certificate

Keywords

TELOMERASEQ2

PAPAVERINEQ3

CANCERQ3

ALKALOIDSQ2

Abstract

Introduction: Telomere maintenance is essential for the continued proliferation of dividing cells, and is implicated in chromosome stability and cell immortalization. TELOMERASE activity, that allows CANCER cells to maintain their telomeric DNA for an indefinite replicative capacity, is an attractive target against CANCER. A well known benzylisoquinoline alkaloid, PAPAVERINE, we focused on to evaluate its antiproliferaive effects on breast CANCER MCF7 cells.Methods: Cytotoxicity of the commercially available pure compound PAPAVERINE HCl (Sigma) was determined by MTT assay. A modified quantitative real-time polymerase chain reaction (PCR) -based TELOMERASE repeat amplification protocol (TRAP) was used to estimate relative TELOMERASE activity in PAPAVERINE-treated cells in comparison with the untreated control cells. Relative expression level of the catalytic subunit of TELOMERASE (hTERT) gene was estimated using real time reverse transcription-PCR (RT-PCR).Results: IC50 concentration of PAPAVERINE after 48 hours treatment was measured to 120 micromolar At this concentration TELOMERASE activity showed a considerable decrease (almost 70% in comparison with untreated control cells), in a concentration dependent manner. Quantitative real-time RT-PCR experiments indicated a similar reduction in transcription level of hTERT gene under treatment with PAPAVERINE.Conclusion: PAPAVERINE is a potent natural compound in suppression of CANCER cell immortality most probably by antiTELOMERASE activity. It is a valuable putative compound for further development of promising anti-cancer agents.

Cites

No record.

References

No record.

Cite

APA: Copy

KAZEMI NOUREINI, S., NABIUNI, M., NOORI, Z.A., & KHOSROJERDI, F.. (2013). INVESTIGATION OF TELOMERASE ACTIVITY AND HTERT GENE EXPRESSION IN MCF7 CELLS TREATED WITH PAPAVERINE. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 20(2 (68)), 121-132. SID. https://sid.ir/paper/393684/en

Vancouver: Copy

KAZEMI NOUREINI S., NABIUNI M., NOORI Z.A., KHOSROJERDI F.. INVESTIGATION OF TELOMERASE ACTIVITY AND HTERT GENE EXPRESSION IN MCF7 CELLS TREATED WITH PAPAVERINE. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2013;20(2 (68)):121-132. Available from: https://sid.ir/paper/393684/en

IEEE: Copy

S. KAZEMI NOUREINI, M. NABIUNI, Z.A. NOORI, and F. KHOSROJERDI, “INVESTIGATION OF TELOMERASE ACTIVITY AND HTERT GENE EXPRESSION IN MCF7 CELLS TREATED WITH PAPAVERINE,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 20, no. 2 (68), pp. 121–132, 2013, [Online]. Available: https://sid.ir/paper/393684/en

Related Journal Papers