Phenotypic and Genotypic Study of Antibiotic Resistance among Escherichia coli Isolates from Human Urinary Infection Cases in Bojnord Province

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Amiri Mohadeseh; FARZIN Hamidreza; Jamshidian Mojaver Majid

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Journal: Avicenna Journal of Clinical Medicine Year:2019 | Volume:26 | Issue:3 (93) Page(s): 173-180

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Title

Phenotypic and Genotypic Study of Antibiotic Resistance among Escherichia coli Isolates from Human Urinary Infection Cases in Bojnord Province

Author(s)

Amiri Mohadeseh | FARZIN Hamidreza | Jamshidian Mojaver Majid | Issue Writer Certificate

Keywords

AntibiogramQ2

Antibiotic ResistanceQ1

Urinary Tract InfectionQ2

Abstract

Background and Objective: Bacterial agents are the most common causes of urinary infection with Escherichia coli as the major causative organism. Accordingly, the current study aimed to determine the Antibiotic Resistance among Escherichia coli isolates from human urinary infection cases. Materials and Methods: The current experimental study was carried out on 50 specimens of positive cultures with Urinary Tract Infection referred to Imam Reza Hospital Laboratory in Bojnord, Iran. The resistance and susceptibility of the isolates were assessed using disc diffusion method. Moreover, the presence of tetA, blaTEM, Sul1, aac(3)-IV, and aadA1 gens was examined using molecular methods with specific primers. Results: Prevalence of Antibiotic Resistance to ampicillin, cotrimaxazole, levofloxacin, nalidixic acid, ciprofloxacin, gentamicin, and nitrofurantoin was measured at 84%, 60%, 60%, 52%, 44%, 22%, and 6%, respectively. in total, 50 E. coli strains were isolated were examined to determine blaTEM, aac(3)-IV, tetA, Sul, and aadA1 genes using polymerase chain reaction(PCR). Based on the obtained results, the frequency of blaTEM, aac(3)-IV, tetA, Sul1, aac(3)-IV, and aadA1 genes was reported as 24%, 12%, 10%, 8%, and 14%, respectively. Conclusion: Disk diffusion agar method can be used as a primary screening method to determine antibiotic susceptibility for Escherichia coli isolates separated from Urinary Tract Infections. In addition, genotypic method can be implemented for the accurate evaluation of the resistance of the isolates.

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APA: Copy

Amiri, Mohadeseh, FARZIN, Hamidreza, & Jamshidian Mojaver, Majid. (2019). Phenotypic and Genotypic Study of Antibiotic Resistance among Escherichia coli Isolates from Human Urinary Infection Cases in Bojnord Province. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 26(3 (93) ), 173-180. SID. https://sid.ir/paper/394910/en

Vancouver: Copy

Amiri Mohadeseh, FARZIN Hamidreza, Jamshidian Mojaver Majid. Phenotypic and Genotypic Study of Antibiotic Resistance among Escherichia coli Isolates from Human Urinary Infection Cases in Bojnord Province. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2019;26(3 (93) ):173-180. Available from: https://sid.ir/paper/394910/en

IEEE: Copy

Mohadeseh Amiri, Hamidreza FARZIN, and Majid Jamshidian Mojaver, “Phenotypic and Genotypic Study of Antibiotic Resistance among Escherichia coli Isolates from Human Urinary Infection Cases in Bojnord Province,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 26, no. 3 (93) , pp. 173–180, 2019, [Online]. Available: https://sid.ir/paper/394910/en

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