Design of nanoparticle loaded LHRH-A2 hormone controlled delivery system for using in aquatics

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Noori Mehrnaz; FALAHATKAR BAHRAM; SHAHSAVARI SHADAB

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Journal: AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY Year:2019 | Volume:7 | Issue:1 Page(s): 161-180

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Title

Design of nanoparticle loaded LHRH-A2 hormone controlled delivery system for using in aquatics

Author(s)

Noori Mehrnaz | FALAHATKAR BAHRAM | SHAHSAVARI SHADAB | Issue Writer Certificate

Keywords

LHRH-A2Q1

NanochitosanQ1

Slow Release SystemQ1

HormoneQ1

MaturationQ2

Abstract

The present study aims to develop a new nanoparticle-based system. The natural polymer chitosan for nanoparticles and luteinizing Hormone releasing Hormone analogue (LHRH-A2) were used as a Hormone which utilizes for fish artificial propagation. The ionic gelation method was applied to prepare the chitosan nanoparticles. The design in the present study was an experimental D-optimal response-surface method. The variables were the concentration of the Hormone, the concentration ratio of chitosan/tripolyphosphate and Hormone-chitosan solution pH. The nanoparticles were determined morphologically through the scanning electron microscopy (SEM). In order to check the size, the zeta and PdI particles, dynamic light scattering instrument, and infrared spectroscopy were used. Moreover, regarding the nanoparticles’ structure formation and detection of thermal resistance, the differential scanning calorimetry was applied. The results displayed the nanoparticles optimal size was 108 nm, the zeta potential was 16. 2μ V and the particle size distribution index was 0. 274. The result of the in vitro release of the prepared nanoparticles revealed that the percentage of released Hormone from the Nanochitosan was 80. 14% within 48 hours. The SEM image showed the segregated and non-aggregated nanoparticles in sub-spherical smooth morphology. Furthermore, it is revealed that the high thermal stability of Hormone nanoparticles at a temperature up to 400° C. This system can be used as a suitable injection carrier for slow-release Hormone system in induction of various species of fish such as sturgeons.

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APA: Copy

Noori, Mehrnaz, FALAHATKAR, BAHRAM, & SHAHSAVARI, SHADAB. (2019). Design of nanoparticle loaded LHRH-A2 hormone controlled delivery system for using in aquatics. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, 7(1 ), 161-180. SID. https://sid.ir/paper/394924/en

Vancouver: Copy

Noori Mehrnaz, FALAHATKAR BAHRAM, SHAHSAVARI SHADAB. Design of nanoparticle loaded LHRH-A2 hormone controlled delivery system for using in aquatics. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY[Internet]. 2019;7(1 ):161-180. Available from: https://sid.ir/paper/394924/en

IEEE: Copy

Mehrnaz Noori, BAHRAM FALAHATKAR, and SHADAB SHAHSAVARI, “Design of nanoparticle loaded LHRH-A2 hormone controlled delivery system for using in aquatics,” AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, vol. 7, no. 1 , pp. 161–180, 2019, [Online]. Available: https://sid.ir/paper/394924/en

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