FREQUENCY DETERMINATION OF HLA-DRB1 AND HLA-DQB1 ALLELES IN CHILDREN WITH PRIMARY VESICOURETERAL REFLUX

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BAZRAFSHANI MOHAMMADREZA; PARVARESH SAEIDEH; SADEGHI LOTFABADI ALI; HOSSEINI FATEMESADAT

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Journal: Tehran University Medical Journal Year:2014 | Volume:72 | Issue:9 Page(s): 601-607

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Title

FREQUENCY DETERMINATION OF HLA-DRB1 AND HLA-DQB1 ALLELES IN CHILDREN WITH PRIMARY VESICOURETERAL REFLUX

Author(s)

BAZRAFSHANI MOHAMMADREZA | PARVARESH SAEIDEH | SADEGHI LOTFABADI ALI | HOSSEINI FATEMESADAT | Issue Writer Certificate

Keywords

HLA-DQB1Q2

HLA-DRB1Q3

HUMAN LEUKOCYTE ANTIGENQ2

VESICOURETERAL REFLUXQ2

Abstract

Background: VESICOURETERAL REFLUX (VUR) is the retrograde flow of urine from the bladder into the ureter and toward the kidney. VESICOURETERAL REFLUX is the most common inherited disease in urogenital system. Primary VUR is the most common urological anomaly in children and it has been reported in 30-50% of those who present with urinary tract infection (UTI). The association of VESICOURETERAL REFLUX, urinary tract infection and renal damage is well known. Current methods for VESICOURETERAL REFLUX diagnosis are unpleasant. Therefore, HUMAN LEUKOCYTE ANTIGEN system not only might help to detect causative gene but also would assists to establish better prognoses tests of this disease. In this study, the relationship between VESICOURETERAL REFLUX and HLA-DRB1 and HLA-DQB1 genes were investigated.Methods: This study applied on forty VESICOURETERAL REFLUX confirmed children from Kerman province, Iran. These children have been admitted to the Afzalipour Hospital for UTI and primary VUR for them was proved by voiding cystourethrogram (VCUG). Also, forty children without any VUR sign as control group. DNA was extracted from the whole blood sample and was amplified using sequence-specific priming polymerase chain reaction (PCR-SSP) method. Finally PCR products were evaluated by electrophoresis in 1.5% agarose gel and frequency of alleles and haplotypes were compared by Chi-square test. Significance level was assumed at P<0.05.Results: Low-resolution HLA typing showed the frequency of the HLA-DR17 antigen was significantly increased in VESICOURETERAL REFLUX children compared to control group (P=0.039). On the other hand HLA-DR16 was significantly decreased in VESICOURETERAL REFLUX group. Also, frequency of HLA-DQ2 was significantly higher in patients compared to control group (P=0.002). DRB1 (11, 17) and DQ (2, 7) haplotypes were also higher in VESICOURETERAL REFLUX patients (P=0.027, P=0.01).Conclusion: The HLA cluster might affect on susceptibility to VESICOURETERAL REFLUX especially by locus which located close to HLA-DRB1 and HLA-DQB1 genes. This study demonstrates for the first time in Iran. However, further extensive researches with a large number of samples from different populations and ethnicities are required to validate the results obtained in this study.

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APA: Copy

BAZRAFSHANI, MOHAMMADREZA, PARVARESH, SAEIDEH, SADEGHI LOTFABADI, ALI, & HOSSEINI, FATEMESADAT. (2014). FREQUENCY DETERMINATION OF HLA-DRB1 AND HLA-DQB1 ALLELES IN CHILDREN WITH PRIMARY VESICOURETERAL REFLUX. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 72(9), 601-607. SID. https://sid.ir/paper/39528/en

Vancouver: Copy

BAZRAFSHANI MOHAMMADREZA, PARVARESH SAEIDEH, SADEGHI LOTFABADI ALI, HOSSEINI FATEMESADAT. FREQUENCY DETERMINATION OF HLA-DRB1 AND HLA-DQB1 ALLELES IN CHILDREN WITH PRIMARY VESICOURETERAL REFLUX. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2014;72(9):601-607. Available from: https://sid.ir/paper/39528/en

IEEE: Copy

MOHAMMADREZA BAZRAFSHANI, SAEIDEH PARVARESH, ALI SADEGHI LOTFABADI, and FATEMESADAT HOSSEINI, “FREQUENCY DETERMINATION OF HLA-DRB1 AND HLA-DQB1 ALLELES IN CHILDREN WITH PRIMARY VESICOURETERAL REFLUX,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 72, no. 9, pp. 601–607, 2014, [Online]. Available: https://sid.ir/paper/39528/en

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