Molecular Analysis of AmpC Genomic Polymorphism in Shigella Sonnei Isolated from CLinical Specimens by PCR-RFLP Method

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ABASSI A.; AMINI K.

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Journal: JOURNAL OF ADVANCED BIOMEDICAL SCIENCES Year:2020 | Volume:10 | Issue:2 Page(s): 2348-2354

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Title

Molecular Analysis of AmpC Genomic Polymorphism in Shigella Sonnei Isolated from CLinical Specimens by PCR-RFLP Method

Author(s)

ABASSI A. | AMINI K. | Issue Writer Certificate

Keywords

Shigella sonneiQ1

ampC beta-lactamase geneQ1

RFLP-PCRQ1

Abstract

Background & Objective: Shigella is a gram-negative bacillus that has caused serious infection problems in developed and developing countries. The existence of beta-lactamase genes such as AmpC in Shigella species in one country has led to a variety of antibiotic resistance patterns, which can have very serious health risks for the community. The aim of this study was to investigate the prevalence of AmpC β-lactamase gene in S. sonnei strains and determine the prevalence of a spot mutation in this gene by RFLP method. Materials & methods: Sixty strains of S. sonei were detected using biochemical and microbiological tests. Subsequently, the amplification of ampC gene was performed by PCR method. Then, a point mutation analysis on this gene was performed on ampC gene positive strains. RFLP technique was performed using Hinf1 enzyme. Results: Among 60 isolates, 30 (50%) isolates were positive for ampC beta-lactamase gene. Furthermore, A to G point mutation was detected in 11 (37%) ampC gene carrying isolates. Conclusion: The results of this study showed an increasing rate in the prevalence of ampC gene and noticeable prevalence of A to G point mutation in the strains carrying this gene. This mutation may help increase resistance to cephalosporins.

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APA: Copy

ABASSI, A., & AMINI, K.. (2020). Molecular Analysis of AmpC Genomic Polymorphism in Shigella Sonnei Isolated from CLinical Specimens by PCR-RFLP Method. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 10(2 ), 2348-2354. SID. https://sid.ir/paper/397202/en

Vancouver: Copy

ABASSI A., AMINI K.. Molecular Analysis of AmpC Genomic Polymorphism in Shigella Sonnei Isolated from CLinical Specimens by PCR-RFLP Method. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2020;10(2 ):2348-2354. Available from: https://sid.ir/paper/397202/en

IEEE: Copy

A. ABASSI, and K. AMINI, “Molecular Analysis of AmpC Genomic Polymorphism in Shigella Sonnei Isolated from CLinical Specimens by PCR-RFLP Method,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 10, no. 2 , pp. 2348–2354, 2020, [Online]. Available: https://sid.ir/paper/397202/en

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