IDENTIFICATION OF IPA AND STX GENES AND THEIR ANTIBIOTIC RESISTANCE PATTERN IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES USING MULTIPLEX-PCR

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GANJIPOUR M.; AMINI K.; MORADLI G.

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Journal: SADRA MEDICAL SCIENCES JOURNAL Year:2016 | Volume:4 | Issue:2 Page(s): 127-135

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Information Journal Paper

Title

IDENTIFICATION OF IPA AND STX GENES AND THEIR ANTIBIOTIC RESISTANCE PATTERN IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES USING MULTIPLEX-PCR

Author(s)

GANJIPOUR M. | AMINI K. | MORADLI G. | Issue Writer Certificate

Keywords

IPA GENEQ1

STX GENEQ1

SHIGELLA SONNEIQ1

MULTIPLEX-PCRQ1

ANTIBIOTIC RESISTANCEQ1

Abstract

Background: Shigella is responsible for shigellosis or bacillary dysentery, which is transmitted by contaminated food. Invasion Plasmid Antigens (Ipa) gene leads to intra- or extra-cellular spreading of Shigella. The current study aimed to identify Ipa and STX GENEs in SHIGELLA SONNEI isolated from clinical samples by MULTIPLEX-PCR and determine their ANTIBIOTIC RESISTANCE profile.Methods: In this study, 150 stool samples were collected from different treatment centers in Tehran. After culture-based microbiological and biochemical tests, 60 SHIGELLA SONNEI species were identified. Antibiotic susceptibility tests were performed by disk diffusion method according to CLSI guideline. Then, MULTIPLEX-PCR assay was employed to identify stx and IPA GENEs.Results: The results showed that the highest and lowest resistance was related to co-trimoxazole (n=60, 100%) and ceftriaxone (n=10, 16.7%), respectively. Out of the 60 isolates, 57 strains (95%) were positive for ipaH gene and other virulence genes were not detected in any of the samples.Conclusion: ipaH, due to presence in the majority of Shigella strains, can be a candidate to identify this organism. It seems that indiscriminate use of antibiotics is one of the causes of ANTIBIOTIC RESISTANCE in Shigella species.

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APA: Copy

GANJIPOUR, M., AMINI, K., & MORADLI, G.. (2016). IDENTIFICATION OF IPA AND STX GENES AND THEIR ANTIBIOTIC RESISTANCE PATTERN IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES USING MULTIPLEX-PCR. SADRA MEDICAL SCIENCES JOURNAL, 4(2), 127-135. SID. https://sid.ir/paper/238780/en

Vancouver: Copy

GANJIPOUR M., AMINI K., MORADLI G.. IDENTIFICATION OF IPA AND STX GENES AND THEIR ANTIBIOTIC RESISTANCE PATTERN IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES USING MULTIPLEX-PCR. SADRA MEDICAL SCIENCES JOURNAL[Internet]. 2016;4(2):127-135. Available from: https://sid.ir/paper/238780/en

IEEE: Copy

M. GANJIPOUR, K. AMINI, and G. MORADLI, “IDENTIFICATION OF IPA AND STX GENES AND THEIR ANTIBIOTIC RESISTANCE PATTERN IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES USING MULTIPLEX-PCR,” SADRA MEDICAL SCIENCES JOURNAL, vol. 4, no. 2, pp. 127–135, 2016, [Online]. Available: https://sid.ir/paper/238780/en

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