Effect of glucose on improvement of BM-MSC differentiation into cardiomyocyte-like cells

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Raisolsadati Sara; MARJANI ABDOLJALAL; Khajeniazi Safoura

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Journal: JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:21 | Issue:3 (71) Page(s): 41-48

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Title

Effect of glucose on improvement of BM-MSC differentiation into cardiomyocyte-like cells

Author(s)

Raisolsadati Sara | MARJANI ABDOLJALAL | Khajeniazi Safoura | Issue Writer Certificate

Keywords

CardiomyocyteQ2

Mesenchymal stem cellQ2

GlucoseQ2

5-AzacytidineQ1

Cardiac-specific markersQ1

Abstract

Background and Objective: Cardiovascular diseases and heart failure are major diseases in developed countries. Stem cells showed specific features to play an important role in heart disease treatment. One of the most common compounds has been used to induce differentiation of stem cells into Cardiomyocyte is 5-Azacytidine. Medium contents of cell culture such as different Glucose concentrations also influence on morphology and function of final differentiated cells. This study was done to evaluate the effect of Glucose on improvement of BM-MSC differentiation into Cardiomyocyte-like cells. Methods: In this experimental study, effect of two different Glucose concentrations (5 and 25mM) on the Mesenchymal stem cells differentiation (MSCs) to Cardiomyocytes during 21 days was evaluated. Bone marrow MSCs (BM MSCs) seeded in differentiation medium which treated with 5-aza and 5 & 25mM Glucose concentrations. In next step, total RNA was extracted and cDNA synthesis was carried out. Finally, quantitative polymerase chain reaction (Q-PCR) was done to determine level of Cardiac-specific markers during differentiation process including Connexin43, α-cardiac actin, TroponinT and TroponinI. Results: Level of Cardiac-specific markers during differentiation of Mesenchymal stem cells to Cardiomyocyte including Connexin43, α-cardiac actin, TroponinT and TroponinI in 5 and 25 mM of Glucose concentration was diferent, but this diference was not significant. Conclusion: Our results showed that two concentrations of Glucose (5, 25mM) have no remarkable effect on the expression of cardiac markers during differentiation of bone marrow Mesenchymal stem cells to Cardiomyocyte.

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APA: Copy

Raisolsadati, Sara, MARJANI, ABDOLJALAL, & Khajeniazi, Safoura. (2019). Effect of glucose on improvement of BM-MSC differentiation into cardiomyocyte-like cells. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, 21(3 (71) ), 41-48. SID. https://sid.ir/paper/397671/en

Vancouver: Copy

Raisolsadati Sara, MARJANI ABDOLJALAL, Khajeniazi Safoura. Effect of glucose on improvement of BM-MSC differentiation into cardiomyocyte-like cells. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;21(3 (71) ):41-48. Available from: https://sid.ir/paper/397671/en

IEEE: Copy

Sara Raisolsadati, ABDOLJALAL MARJANI, and Safoura Khajeniazi, “Effect of glucose on improvement of BM-MSC differentiation into cardiomyocyte-like cells,” JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, vol. 21, no. 3 (71) , pp. 41–48, 2019, [Online]. Available: https://sid.ir/paper/397671/en

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