INDENTIFICATION OF LEISHMANIA SPECIES USING MONOCLONAL ANTIBODIES IN ISFAHAN

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HEJAZI H.; NASRIFAR P.; JAMALI SOUDABEH; JAHANGIRNEZHAD A.A.; KHAMESIPOUR A.

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Journal: Iranian Journal of Dermatology Year:2000 | Volume:4 | Issue:1 (13) Page(s): 7-11

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Title

INDENTIFICATION OF LEISHMANIA SPECIES USING MONOCLONAL ANTIBODIES IN ISFAHAN

Author(s)

Keywords

LEISHMANIAQ3

ELISAQ4

MONOCLONAL ANTIBODYQ4

Abstract

Background: Isfahan is one of the major foci of cutaneous LEISHMANIAsis in Iran. According to the epidemioligical studies, both urban and rural forms have been reported in Isfahan. Accurate identification and characterization of LEISHMANIA is essential for the control of the disease. Several different molecular techniques for LEISHMANIA identification are developed including use of monoclonal antibodies.Objective: In the present study specific monoclonal antibodies against L.major (T1, T9), L.tropica (T10, T11) and L.Donovani (D2) were used to identify and characterize the LEISHMANIA parasites isolated from patients with cutaneous LEISHMANIAsis in Isfahan. Patients & Methods: Samples were obtained from borders of lesions suspicious of LEISHMANIAsis in 298 patients, stained with Giemsa and cultured in NNN and RPMI 1640 media supplemented with %10 fetal colf serum. The promastigotes were then purified, counted and used as antigen in ELISA test. Results: Mass production of promastigotes was successful in 120 cases. L.major was identified as the causative agent of the disease in 100 cases and L.tropica in 8 patients. In 12 cases the results were inconclusive as the antigens reacted with either 2 or 3 specific monoclonal antibodies. Conclusion: L.major is the most common cause of LEISHMANIAsis in Isfahan.

Cites

EXPENSE OF TREATMENT OF CUTANEOUS LEISHMANIASIS IN ISFAHAN PROVINCE IN 1999

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APA: Copy

HEJAZI, H., NASRIFAR, P., JAMALI, SOUDABEH, JAHANGIRNEZHAD, A.A., & KHAMESIPOUR, A.. (2000). INDENTIFICATION OF LEISHMANIA SPECIES USING MONOCLONAL ANTIBODIES IN ISFAHAN. IRANIAN JOURNAL OF DERMATOLOGY, 4(1 (13)), 7-11. SID. https://sid.ir/paper/397978/en

Vancouver: Copy

HEJAZI H., NASRIFAR P., JAMALI SOUDABEH, JAHANGIRNEZHAD A.A., KHAMESIPOUR A.. INDENTIFICATION OF LEISHMANIA SPECIES USING MONOCLONAL ANTIBODIES IN ISFAHAN. IRANIAN JOURNAL OF DERMATOLOGY[Internet]. 2000;4(1 (13)):7-11. Available from: https://sid.ir/paper/397978/en

IEEE: Copy

H. HEJAZI, P. NASRIFAR, SOUDABEH JAMALI, A.A. JAHANGIRNEZHAD, and A. KHAMESIPOUR, “INDENTIFICATION OF LEISHMANIA SPECIES USING MONOCLONAL ANTIBODIES IN ISFAHAN,” IRANIAN JOURNAL OF DERMATOLOGY, vol. 4, no. 1 (13), pp. 7–11, 2000, [Online]. Available: https://sid.ir/paper/397978/en

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