EVALUATION OF TWO COMMERCIAL QUALITATIVE AND SEMI QUANTITATIVE ELISA METHODS FOR DIAGNOSIS OF ACUTE HUMAN LEPTOSPIROSIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HONARMAND H.; ESHRAGHI S.; KHORAMIZADEH MOHAMMAD REZA; HARESKEERL R.; MANSOUR GHANAEI F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES Year:2006 | Volume:15 | Issue:59 Page(s): 26-31

Download Full-Text

Persian Verion

View:

911

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF TWO COMMERCIAL QUALITATIVE AND SEMI QUANTITATIVE ELISA METHODS FOR DIAGNOSIS OF ACUTE HUMAN LEPTOSPIROSIS

Author(s)

Keywords

ENZYMEQ2

LINKED IMMUNOSORBENT ASSAYQ3

LEPTOSPIROSISQ3

Abstract

Introduction: LEPTOSPIROSIS is most widespread Zoonosis in the world, especially in Tropical and temperate regions with moist climate. Diagnosis of LEPTOSPIROSIS according to clinical symptoms is difficult and uncertain due to lack of specific sign (s). Leptospira is a fastidious bacterium. Isolation of these bacteria by culture is difficult, time consuming and hence doses not contribute to an early diagnosis. Specific antibodies against Leptospira appear from 6th day of disease onset. The Microscopic Agglutination Test (MAT) is most reliable assay but generally requires paired sera for detection of seroconversion and is considered too complex for routine use.Objective: We performed this study to final a simple and reliable method for diagnosis of acute human LEPTOSPIROSIS. ELISA is another common method for diagnosis of LEPTOSPIROSIS.Materials and Methods: In summer of 2004, we examined 282single sera of patients who were suspected of LEPTOSPIROSIS by a commercial none quantities and an in-house semi quantative ELISA assays and compared their results with MAT.Results: Mean time of first taking blood samples were 6:36 after onset of symptoms. All specimens with titers ≥640 against a pathogenic serovar in MAT were regarded as confirmed LEPTOSPIROSIS(104 from 282). All specimens which were positive in any IgM-ELISA assays were compared with the results of MAT. In our study, sensitivity, specify, positive and negative predictive values of in house ELISA were 89.4%, 87%, 80.2% and 93.4% consequently but were 87.5%, 41.5%, 44.6% and 85% for comerical IgM-ELISA assay consequently.Conclusion: The results of our study show that IgM- ELISA assay is a reliable and sensitive method for diagnosis of acute LEPTOSPIROSIS and also show that in house semi quantative IgM ELISA was more specific and commercial qualitative IgM- ELISA was more sensitive.

Cites

No record.

References

No record.

Cite

APA: Copy

HONARMAND, H., ESHRAGHI, S., KHORAMIZADEH, MOHAMMAD REZA, HARESKEERL, R., & MANSOUR GHANAEI, F.. (2006). EVALUATION OF TWO COMMERCIAL QUALITATIVE AND SEMI QUANTITATIVE ELISA METHODS FOR DIAGNOSIS OF ACUTE HUMAN LEPTOSPIROSIS. JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES, 15(59), 26-31. SID. https://sid.ir/paper/39946/en

Vancouver: Copy

HONARMAND H., ESHRAGHI S., KHORAMIZADEH MOHAMMAD REZA, HARESKEERL R., MANSOUR GHANAEI F.. EVALUATION OF TWO COMMERCIAL QUALITATIVE AND SEMI QUANTITATIVE ELISA METHODS FOR DIAGNOSIS OF ACUTE HUMAN LEPTOSPIROSIS. JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2006;15(59):26-31. Available from: https://sid.ir/paper/39946/en

IEEE: Copy

H. HONARMAND, S. ESHRAGHI, MOHAMMAD REZA KHORAMIZADEH, R. HARESKEERL, and F. MANSOUR GHANAEI, “EVALUATION OF TWO COMMERCIAL QUALITATIVE AND SEMI QUANTITATIVE ELISA METHODS FOR DIAGNOSIS OF ACUTE HUMAN LEPTOSPIROSIS,” JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES, vol. 15, no. 59, pp. 26–31, 2006, [Online]. Available: https://sid.ir/paper/39946/en

Related Journal Papers