COMPARISON ON EFFECT OF ZINC ON VIABILITY AND MORPHOLOGY OF RAJI AND MOLT-4 CELL-LINE

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TOKMEH DASHI H.; OUSATI ASHTIANI F.; POURFATH ELAH A.A.

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Journal: Journal of Guilan University of Medical Sciences Year:2004 | Volume:13 | Issue:51 Page(s): 32-37

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Title

COMPARISON ON EFFECT OF ZINC ON VIABILITY AND MORPHOLOGY OF RAJI AND MOLT-4 CELL-LINE

Author(s)

TOKMEH DASHI H. | OUSATI ASHTIANI F. | POURFATH ELAH A.A. | Issue Writer Certificate

Keywords

B-LYMPHAYTESQ3

T-LYMPHOCYTESQ3

ZINCQ3

Abstract

Introduction: ZINC has important effects on structural and functional activities of many proteins and enzymes, especially in regulation of immune system. Objective: This study was carried out to examine the in vitro effects of different concentrations of ZINC on viability and morphology of T-Lymphoid (Molt-4) and B-Lymphoid (Raji) cell line. Materials and Methods: In this study, the cell line was exposed to different concentrations of ZINC (100 µM to 500 µM) followed by incubation (37°C, 5%Co2) at various time points (12 to 72 hrs). The cells were then evaluated with trypan blue exclusion dye , and Wright-Gimsa staining.Results: The results of this study showed almost different responses to different amounts of ZINC by the T and B cell line. Concentrations less than 100 µM of ZINC at different incubation time points had little to no effects on cell line when compared to the controls. Higher concentrations of ZINC (>100 µM) diminished cell viability to 70% at 12 hrs and less than 50% at 24 to 72 hrs of incubation times.Conclusion: We concluded that ZINC shows cytotoxic effects (Raji & Molt-4) depending on the concentrations used. At higher concentrations, it exerts cytotoxic effects.

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APA: Copy

TOKMEH DASHI, H., OUSATI ASHTIANI, F., & POURFATH ELAH, A.A.. (2004). COMPARISON ON EFFECT OF ZINC ON VIABILITY AND MORPHOLOGY OF RAJI AND MOLT-4 CELL-LINE . JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES, 13(51), 32-37. SID. https://sid.ir/paper/40050/en

Vancouver: Copy

TOKMEH DASHI H., OUSATI ASHTIANI F., POURFATH ELAH A.A.. COMPARISON ON EFFECT OF ZINC ON VIABILITY AND MORPHOLOGY OF RAJI AND MOLT-4 CELL-LINE . JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2004;13(51):32-37. Available from: https://sid.ir/paper/40050/en

IEEE: Copy

H. TOKMEH DASHI, F. OUSATI ASHTIANI, and A.A. POURFATH ELAH, “COMPARISON ON EFFECT OF ZINC ON VIABILITY AND MORPHOLOGY OF RAJI AND MOLT-4 CELL-LINE ,” JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES, vol. 13, no. 51, pp. 32–37, 2004, [Online]. Available: https://sid.ir/paper/40050/en

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