INHIBITORY EFFECT OF EICOSAPENTAENOIC ACID (EPA) ON SFLT-1 (SOLUBLE VEGF RECEPTOR-1) EXPRESSION IN TROPHOBLAST TUMOR CELL LINE JEG-3

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ZAVARREZA J.; SHEIKHHA M.H.; KALANTAR M.; BAHRAMI M.; AFSHARI Z.; ZARE F.

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2013 | Volume:21 | Issue:1 (90) Page(s): 53-60

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Title

INHIBITORY EFFECT OF EICOSAPENTAENOIC ACID (EPA) ON SFLT-1 (SOLUBLE VEGF RECEPTOR-1) EXPRESSION IN TROPHOBLAST TUMOR CELL LINE JEG-3

Author(s)

Keywords

EPAQ2

HYPOXIA.SFLTQ3

1Q2

JEGQ2

3Q2

PREECLAMPSIAQ2

Abstract

Introduction: Previous studies have shown altered levels of n-3LCPUFA in the pathophysiologcal conditions such as PREECLAMPSIA. Also elevated expression of sFlt-1 in PREECLAMPSIA plays a major role in the pathogenesis of this serious disorder especially in reduced placental oxygenation. The present study examines the hypothesis that Eicosapentaenoic acid (EPA; 20:5), an omega-3 long-chain polyunsaturated fatty acids (n-3LCPUFAs), may attenuate sFlt-1 gene and protein expression in JEG-3 cells treated with induced hypoxia-like conditions by (DMOG) -induced hypoxia-like conditions.Methods: JEG-3 cells were pretreated with DMOG incubated with EPA. Protein expression of sFlt-1 was measured by enzyme-linked immunosorbent assay (ELISA). Messenger RNA expressions of sFlt-1 was determined by and RT Real Time-PCR.Results: Our results showed that incubation of JEG-3 cells with DMOG cause a significant elevation in mRNA levels and protein secretion of sFlt-1 (P < 0.05). In contrast, EPA decreased the mRNA expression and protein secretion of sFlt-1 (P < 0.05). Also mRNA expression and protein secretion of sFlt-1 inhibited cells treated by both EPA and DMOG (P=0.261, P =0.077 respectively).Conclusion: These findings confirm previous studies that hypoxia caused elevation in sFlt-1 gene expression and protein secretion. Also our studies reveal that effects of n-3 fatty acids in restraining PREECLAMPSIA complications may be mediated by suppressing the gene expression and protein secretion of sFlt-1 under hypoxia conditions. This data provide evidence that n-3 LCPUFA can exert its effects through inhibition of the HIF pathway.

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APA: Copy

ZAVARREZA, J., SHEIKHHA, M.H., KALANTAR, M., BAHRAMI, M., AFSHARI, Z., & ZARE, F.. (2013). INHIBITORY EFFECT OF EICOSAPENTAENOIC ACID (EPA) ON SFLT-1 (SOLUBLE VEGF RECEPTOR-1) EXPRESSION IN TROPHOBLAST TUMOR CELL LINE JEG-3. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 21(1 (90)), 53-60. SID. https://sid.ir/paper/401936/en

Vancouver: Copy

ZAVARREZA J., SHEIKHHA M.H., KALANTAR M., BAHRAMI M., AFSHARI Z., ZARE F.. INHIBITORY EFFECT OF EICOSAPENTAENOIC ACID (EPA) ON SFLT-1 (SOLUBLE VEGF RECEPTOR-1) EXPRESSION IN TROPHOBLAST TUMOR CELL LINE JEG-3. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2013;21(1 (90)):53-60. Available from: https://sid.ir/paper/401936/en

IEEE: Copy

J. ZAVARREZA, M.H. SHEIKHHA, M. KALANTAR, M. BAHRAMI, Z. AFSHARI, and F. ZARE, “INHIBITORY EFFECT OF EICOSAPENTAENOIC ACID (EPA) ON SFLT-1 (SOLUBLE VEGF RECEPTOR-1) EXPRESSION IN TROPHOBLAST TUMOR CELL LINE JEG-3,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 21, no. 1 (90), pp. 53–60, 2013, [Online]. Available: https://sid.ir/paper/401936/en

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