Investigation of MexAB-OprM efflux pump gene expression in clinical isolates of pseudomonas aeruginosa isolated from Intensive Care Unit

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Beig Masomeh; ARABESTANI MOHAMMAD REZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2019 | Volume:13 | Issue:2 Page(s): 142-150

Download Full-Text

Persian Verion

View:

407

Download:

Cites:

Information Journal Paper

Title

Investigation of MexAB-OprM efflux pump gene expression in clinical isolates of pseudomonas aeruginosa isolated from Intensive Care Unit

Author(s)

Beig Masomeh | ARABESTANI MOHAMMAD REZA | Issue Writer Certificate

Keywords

Pseudomonas aeruginosaQ2

Real-TimePCRQ1

PCRQ2

MexAB-OprMQ1

Abstract

Background and Aims: Pseudomonas aeruginosa is one of the most important pathogens of nosocomial infections, especially in the ICU (Intensive Care Unit), which has resistance to a wide range of antibiotics, especially Carbapenems. Among the most important resistance mechanisms of this bacteria against carbapenems are MexAB-OprM efflux pump. Therefore, the aim of this study was to evaluate the gene expression of MexAB-OprM efflux pump in clinical isolates of P. aeruginosa that isolated from ICU. Materials and Methods: A total of 33 sampales were isolated from patient in ICU units from different Hamadan hospitals, since november 2018 to May 2019. Antibiotic susceptibility testing was performed using disk diffusion and Minimal Inhibitory Concentration (MIC) methods by Etest for imipenem. Expression levels of MexAB-OprM efflux pump genes were measured by Real-Time PCR. Results: The results of statistical analysis showed that the highest resistance was to Ceftriaxone 21 (63. 63%) and the lowest resistance was to piperacillin, 11 (33. 33%). The results of the MIC of imipenem showed that among off 33 samples isolated from the ICU, 14 (42. 42%) and 19 (57. 57%) isolates were resistant and susceptible, respectively. Increased expression of of MexA, MexB and OprM genes compared with control strain were observed in 20% (4/20), 25% (5/20) and 20% (4/20) of isolates, respectively. Conclusion: Increased expression of MexAB-OprM efflux pump is one of the most common mechanisms in the resistance of P. aeruginosa isolates against Carbapenem antibiotics in different units of hospitals especially intensive care unit. So identification of resistance mechanisms to Carbapenem antibiotics can be useful in controlling and treating such resistant isolates.

Cites

No record.

References

No record.

Cite

APA: Copy

Beig, Masomeh, & ARABESTANI, MOHAMMAD REZA. (2019). Investigation of MexAB-OprM efflux pump gene expression in clinical isolates of pseudomonas aeruginosa isolated from Intensive Care Unit. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 13(2 ), 142-150. SID. https://sid.ir/paper/402160/en

Vancouver: Copy

Beig Masomeh, ARABESTANI MOHAMMAD REZA. Investigation of MexAB-OprM efflux pump gene expression in clinical isolates of pseudomonas aeruginosa isolated from Intensive Care Unit. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2019;13(2 ):142-150. Available from: https://sid.ir/paper/402160/en

IEEE: Copy

Masomeh Beig, and MOHAMMAD REZA ARABESTANI, “Investigation of MexAB-OprM efflux pump gene expression in clinical isolates of pseudomonas aeruginosa isolated from Intensive Care Unit,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 13, no. 2 , pp. 142–150, 2019, [Online]. Available: https://sid.ir/paper/402160/en

Related Journal Papers