EVALUATION OF NANO SILVER PARTICLES’ CYTOTOXICITY ON L929 FIBROBLAST CELLS BY MTT ASSEY: AN IN VITRO STUDY

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SHAHOON HOSSEIN; HAMEDI ROYA; GOLGONIA PANTEA; YADEGARI ZAHRA

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Journal: JOURNAL OF RESEARCH IN DENTAL SCIENCES Year:2011 | Volume:8 | Issue:2 (28) Page(s): 53-59

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Title

EVALUATION OF NANO SILVER PARTICLES’ CYTOTOXICITY ON L929 FIBROBLAST CELLS BY MTT ASSEY: AN IN VITRO STUDY

Author(s)

SHAHOON HOSSEIN | HAMEDI ROYA | GOLGONIA PANTEA | YADEGARI ZAHRA | Issue Writer Certificate

Keywords

NANOQ2

SILVER PARTICLESQ2

CYTOTOXICITYQ2

L929 FIBROBLAST CELLSQ2

PHOTO ABSORPTIONQ2

Abstract

Background and Aim: Antimicrobial effects of NANO SILVER PARTICLES have received enormous attention in medical and dental applications. Despite of wide spread use of NANOsilver there is not enough studies about its side effects on human. Some studies have exhibited CYTOTOXICITY of NANO-silver particles. This research was designed to investigate the cytotoxic effect of NANO SILVER PARTICLES on L929 fibroblasts cells, by MTT assay.Materials and Methods : In this experimental study, NANO SILVER PARTICLES with 5, 10, 20, 30, 40 and 50 ppm concentration were exposed to 10.000 L929 FIBROBLAST CELLS then cell vitality was assessed after 2,24,48 and 72 hours by MTT method. The light absorption was evaluated by Elisa Reader and recorded. Data was subjected to ANOVA test for statistical analysis.Results: In all concentration groups, vitality of cells were the least after 24 hours, but the vitality of cells increased after 48 hours. The increase after 48H was statistically significant (p<0.05). Also concentrations more than 20 ppm in 2,24 and 48 H were significantly cytotoxic for fibroblasts.Conclusion: NANO SILVER PARTICLES at concentrations less than 20 ppm after 72 hours did not have toxic effects.

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APA: Copy

SHAHOON, HOSSEIN, HAMEDI, ROYA, GOLGONIA, PANTEA, & YADEGARI, ZAHRA. (2011). EVALUATION OF NANO SILVER PARTICLES’ CYTOTOXICITY ON L929 FIBROBLAST CELLS BY MTT ASSEY: AN IN VITRO STUDY. JOURNAL OF RESEARCH IN DENTAL SCIENCES, 8(2 (28)), 53-59. SID. https://sid.ir/paper/403022/en

Vancouver: Copy

SHAHOON HOSSEIN, HAMEDI ROYA, GOLGONIA PANTEA, YADEGARI ZAHRA. EVALUATION OF NANO SILVER PARTICLES’ CYTOTOXICITY ON L929 FIBROBLAST CELLS BY MTT ASSEY: AN IN VITRO STUDY. JOURNAL OF RESEARCH IN DENTAL SCIENCES[Internet]. 2011;8(2 (28)):53-59. Available from: https://sid.ir/paper/403022/en

IEEE: Copy

HOSSEIN SHAHOON, ROYA HAMEDI, PANTEA GOLGONIA, and ZAHRA YADEGARI, “EVALUATION OF NANO SILVER PARTICLES’ CYTOTOXICITY ON L929 FIBROBLAST CELLS BY MTT ASSEY: AN IN VITRO STUDY,” JOURNAL OF RESEARCH IN DENTAL SCIENCES, vol. 8, no. 2 (28), pp. 53–59, 2011, [Online]. Available: https://sid.ir/paper/403022/en

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