MOLECULAR CLONING AND BLAST BY GENE BANK OF ALPHA AND BETA SUBUNIT IN LH HORMONE INTO MAMMALIAN SHUTTLE VECTOR PEGFP-N1 USING IRES

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KHORAMGAH MARYAM SADAT; ZARGHAMI NOSRATOLLAH; BANDEHPOUR MOZHGAN; ABBASZADEH HOJATOLLAH; KAZEMI BAHRAM

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Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2013 | Volume:21 | Issue:107 Page(s): 0-0

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Title

MOLECULAR CLONING AND BLAST BY GENE BANK OF ALPHA AND BETA SUBUNIT IN LH HORMONE INTO MAMMALIAN SHUTTLE VECTOR PEGFP-N1 USING IRES

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Keywords

SUB CLONINGQ2

LH HORMONEQ2

EXPRESSION VECTORQ2

Abstract

Background and Objective: The glycoprotein hormone secreted from anterior pituitary gland are heterodimeric, non-covalently consisting of a common α subunit and a hormone-specific b subunit. Due to the lack of adequate access to hormones and the possibility of contamination, this study attempted to clone two subunits of LH HORMONE by using IRES sequence in eukaryotic EXPRESSION VECTOR pEGFP-NI for clinical purposes.Materials and Methods: To clone the LH HORMONE, we designed the subunits alpha and beta sequences, then synthesized the plasmid vector pGEM-BI. The designed sequence was subcloned in the SmaI site of expression plasmid pEGFP-N1. The resulting plasmid was confirmed by restriction enzyme digestion and PCR.Results: PCR and restriction enzyme analysis showed that the plasmid pEGFP-Nl-a-IRES- b contains the correct sequence of the target gene sequences in the gene bank.Conclusion: Since the plasmid has a proper structure, it can be used to transfer into eukaryotic systems and is also appropriate for the evaluation of expression.

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APA: Copy

KHORAMGAH, MARYAM SADAT, ZARGHAMI, NOSRATOLLAH, BANDEHPOUR, MOZHGAN, ABBASZADEH, HOJATOLLAH, & KAZEMI, BAHRAM. (2013). MOLECULAR CLONING AND BLAST BY GENE BANK OF ALPHA AND BETA SUBUNIT IN LH HORMONE INTO MAMMALIAN SHUTTLE VECTOR PEGFP-N1 USING IRES. DANESHVAR MEDICINE, 21(107), 0-0. SID. https://sid.ir/paper/403982/en

Vancouver: Copy

KHORAMGAH MARYAM SADAT, ZARGHAMI NOSRATOLLAH, BANDEHPOUR MOZHGAN, ABBASZADEH HOJATOLLAH, KAZEMI BAHRAM. MOLECULAR CLONING AND BLAST BY GENE BANK OF ALPHA AND BETA SUBUNIT IN LH HORMONE INTO MAMMALIAN SHUTTLE VECTOR PEGFP-N1 USING IRES. DANESHVAR MEDICINE[Internet]. 2013;21(107):0-0. Available from: https://sid.ir/paper/403982/en

IEEE: Copy

MARYAM SADAT KHORAMGAH, NOSRATOLLAH ZARGHAMI, MOZHGAN BANDEHPOUR, HOJATOLLAH ABBASZADEH, and BAHRAM KAZEMI, “MOLECULAR CLONING AND BLAST BY GENE BANK OF ALPHA AND BETA SUBUNIT IN LH HORMONE INTO MAMMALIAN SHUTTLE VECTOR PEGFP-N1 USING IRES,” DANESHVAR MEDICINE, vol. 21, no. 107, pp. 0–0, 2013, [Online]. Available: https://sid.ir/paper/403982/en

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