CLONING, EXPRESSION, AND PURIFICATION OF RECOMBINANT BETA SUBUNIT OF HUMAN TSH PROTEIN AND EVALUATING ITS ANTIGENICITY

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MOHAMMADZADE HADI; KHANSARINEJAD BEHZAD; SADEGHI ABDOLRAHIM; ABTAHI HAMID

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Journal: Journal of Mazandaran University of Medical Sciences Year:2017 | Volume:27 | Issue:147 Page(s): 87-96

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Title

CLONING, EXPRESSION, AND PURIFICATION OF RECOMBINANT BETA SUBUNIT OF HUMAN TSH PROTEIN AND EVALUATING ITS ANTIGENICITY

Author(s)

MOHAMMADZADE HADI | KHANSARINEJAD BEHZAD | SADEGHI ABDOLRAHIM | ABTAHI HAMID | Issue Writer Certificate

Keywords

ELISAQ2

MOLECULAR CLONINGQ2

RECOMBINANT PROTEINQ1

THYROID STIMULATING HORMONEQ2

THYROTROPIN BETA SUBUNITQ2

Abstract

Background and purpose: Measurement of THYROID STIMULATING HORMONE (TSH), usually through RIA and ELISA tests, is very useful for the diagnosis of thyroid disorders. Considering the structural similarity between alpha chain of TSH and the three glycoprotein hormones of luteinizing hormone, follicle stimulating hormone, and chorionic gonadotropin (CG), in this study, we aimed to examine the produced beta-subunit of TSH (TSHb) in terms of antigenicity.Materials and methods: In this study, human TSHb gene was synthesized using recombinant method. The cloning, expression, and purification were performed in Escherichia coli. Thereafter, antigenicity of the protein against antibodies against animal monoclonal TSH was evaluated using Western Blot technique.Results: TSHb gene was cloned and expressed correctly in the bacterial host. The purified protein reacted with the specific antibody in Western Blot. Conclusion: Although recombinant TSHb protein is different from the natural protein in some respects such as structure and spatial shape, it had acceptable antigenicity. On the other hand, it seems that this protein, due to having more TSH epitopes, can be applied in diagnostic kits to enahance the specificity of these tests.

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APA: Copy

MOHAMMADZADE, HADI, KHANSARINEJAD, BEHZAD, SADEGHI, ABDOLRAHIM, & ABTAHI, HAMID. (2017). CLONING, EXPRESSION, AND PURIFICATION OF RECOMBINANT BETA SUBUNIT OF HUMAN TSH PROTEIN AND EVALUATING ITS ANTIGENICITY. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 27(147), 87-96. SID. https://sid.ir/paper/44899/en

Vancouver: Copy

MOHAMMADZADE HADI, KHANSARINEJAD BEHZAD, SADEGHI ABDOLRAHIM, ABTAHI HAMID. CLONING, EXPRESSION, AND PURIFICATION OF RECOMBINANT BETA SUBUNIT OF HUMAN TSH PROTEIN AND EVALUATING ITS ANTIGENICITY. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;27(147):87-96. Available from: https://sid.ir/paper/44899/en

IEEE: Copy

HADI MOHAMMADZADE, BEHZAD KHANSARINEJAD, ABDOLRAHIM SADEGHI, and HAMID ABTAHI, “CLONING, EXPRESSION, AND PURIFICATION OF RECOMBINANT BETA SUBUNIT OF HUMAN TSH PROTEIN AND EVALUATING ITS ANTIGENICITY,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 27, no. 147, pp. 87–96, 2017, [Online]. Available: https://sid.ir/paper/44899/en

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