Rosemary Leaf Extract, a Serum Supplement and Inducer of Human Adipose stem Cells Proliferation in Vitro

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Izadi Tol Sorkhi Maryam; HAJI GHASEM KASHANI MARYAM; GHORBANIAN MOHAMMAD TAGHI

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Paper Information

Journal: Journal of Sabzevar University of Medical Sciences Year:2020 | Volume:26 | Issue:6 Page(s): 677-686

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Information Journal Paper

Title

Rosemary Leaf Extract, a Serum Supplement and Inducer of Human Adipose stem Cells Proliferation in Vitro

Author(s)

Izadi Tol Sorkhi Maryam | HAJI GHASEM KASHANI MARYAM | GHORBANIAN MOHAMMAD TAGHI | Issue Writer Certificate

Keywords

Human Adipose Stem CellsQ1

Rosemary ExtractQ2

ProliferationQ1

SurvivalQ1

Abstract

Introduction: Rosemary leaf extract, as a medicinal plant, can stimulate stem cells Proliferation. Human Adipose Stem Cells (HASCs) are readily available and less harmful accessibility with high therapeutic application. Methods: In this study, fat tissue was isolated from Caesarean women at Damgan Hospital. After mechanical and enzymatic digestion, extracted cells were cultured in α – MEM containing 10% FBS. Stemness of HASCs was confirmed by flow cytometry and their differentiation potential. The Survival rate, Proliferation and neurotrophins genes expression of cells treated with 30, 40, 50, 60 and 70 μ g/ml concentrations of Rosemary Extract containing 40% of carnosic acid for 24 and 48h were evaluated using hoemocytometry, MTT and PCR assays. Results: Cultured HASCs showed spindle and fibroblastic shapes under microscopic study. They expressed positive response to CD44, CD73, CD90, CD105 and negative response to CD34 and CD45markers and differentiated into adipocytes and osteocytes. The Proliferation rate and Survival of cells treated with Rosemary at a concentration of 50 μ g/ml for 48 h were increased significantly as compared to cultured cells in medium without Rosemary, containing serum. Conclusion: Rosemary Extract could be used as a serum supplement to prepare more suitable cells for transplantation.

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References

Adipose-derived stromal cell in regenerative medicine: a review

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APA: Copy

Izadi Tol Sorkhi, Maryam, HAJI GHASEM KASHANI, MARYAM, & GHORBANIAN, MOHAMMAD TAGHI. (2020). Rosemary Leaf Extract, a Serum Supplement and Inducer of Human Adipose stem Cells Proliferation in Vitro. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 26(6 ), 677-686. SID. https://sid.ir/paper/406090/en

Vancouver: Copy

Izadi Tol Sorkhi Maryam, HAJI GHASEM KASHANI MARYAM, GHORBANIAN MOHAMMAD TAGHI. Rosemary Leaf Extract, a Serum Supplement and Inducer of Human Adipose stem Cells Proliferation in Vitro. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2020;26(6 ):677-686. Available from: https://sid.ir/paper/406090/en

IEEE: Copy

Maryam Izadi Tol Sorkhi, MARYAM HAJI GHASEM KASHANI, and MOHAMMAD TAGHI GHORBANIAN, “Rosemary Leaf Extract, a Serum Supplement and Inducer of Human Adipose stem Cells Proliferation in Vitro,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 6 , pp. 677–686, 2020, [Online]. Available: https://sid.ir/paper/406090/en

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