Free and Cell Associated Pertussis Toxin Antigen Quantification to Determine Bordetella Pertussis Appropriate Harvesting Time by ELISA Method

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Helalinasab Mozhgan; NOOFELI MOJTABA; Mohammadbagher Davoud

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Journal: Jundishapur Scientific Medical Journal Year:2019 | Volume:18 | Issue:2 Page(s): 193-203

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Title

Free and Cell Associated Pertussis Toxin Antigen Quantification to Determine Bordetella Pertussis Appropriate Harvesting Time by ELISA Method

Author(s)

Helalinasab Mozhgan | NOOFELI MOJTABA | Mohammadbagher Davoud | Issue Writer Certificate

Keywords

WCP vaccineQ1

PTX antigenQ1

WCP fermenter cultivationQ1

ELISA.Q2

Abstract

Background and Objectives: Duration of whole cell pertussis (WCP) fermenter culture affects the quality and quantity of the Bordetella pertussis PTX production which directly influence in pertussis vaccine potency, and in this way, has a significant consequence on vaccine immunogenicity. The aim of this research was to quantify the cell associated and free pertussis toxin antigen by ELISA.method to determine the optimal and appropriate harvesting time for WCP vaccine production. Material and Methods: The PTX antigen levels were measured in three series of fermenter (350 L) batches (A, B and C) of WCP vaccine strain 509, cultivated in culture media B2 in optical densities of 0. 1, 0. 8, 1. 1, 1. 2, 1. 3. Each sample was quantitatively evaluated in three modes of sediment, supernatant and suspension of WCP vaccine compared to standard pertussis toxin by ELISA.and analyzed using Excel software. Results: According to the analysis of the results which was interpreted based on maximum amount of the PTX antigen anchor to Bordetella pertussis, optimal duration of the fermenter (350 L) culture of WCP strain 509, was determined for 38 to 42 hours at optical density of 1. 2. Conclusion: Due to the disadvantages of turbidity method and the advantages of PTX antigen measurement by ELISA.method (quantitative, qualitative and repeatability), this approach promises a reliable method in order to effectively control he parameters and procedures in the production process, in process quality control (IPQC) and consistency approach of WCP vaccine production.

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APA: Copy

Helalinasab, Mozhgan, NOOFELI, MOJTABA, & Mohammadbagher, Davoud. (2019). Free and Cell Associated Pertussis Toxin Antigen Quantification to Determine Bordetella Pertussis Appropriate Harvesting Time by ELISA Method. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 18(2 ), 193-203. SID. https://sid.ir/paper/406345/en

Vancouver: Copy

Helalinasab Mozhgan, NOOFELI MOJTABA, Mohammadbagher Davoud. Free and Cell Associated Pertussis Toxin Antigen Quantification to Determine Bordetella Pertussis Appropriate Harvesting Time by ELISA Method. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2019;18(2 ):193-203. Available from: https://sid.ir/paper/406345/en

IEEE: Copy

Mozhgan Helalinasab, MOJTABA NOOFELI, and Davoud Mohammadbagher, “Free and Cell Associated Pertussis Toxin Antigen Quantification to Determine Bordetella Pertussis Appropriate Harvesting Time by ELISA Method,” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 18, no. 2 , pp. 193–203, 2019, [Online]. Available: https://sid.ir/paper/406345/en

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