Molecular investigation of the prevalence and detection of enterohemorrhagic Escherichia coli isolated from traditional cheese in Iran

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

malekiparvari Maryam; Parsaeimehr Mahnoosh; STAJI HAMID; Jebellijavan ashkan

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Koomesh Year:2021 | Volume:23 | Issue:1 (81) Page(s): 174-179

Download Full-Text

Persian Verion

View:

241

Download:

Cites:

Information Journal Paper

Title

Molecular investigation of the prevalence and detection of enterohemorrhagic Escherichia coli isolated from traditional cheese in Iran

Author(s)

malekiparvari Maryam | Parsaeimehr Mahnoosh | STAJI HAMID | Jebellijavan ashkan | Issue Writer Certificate

Keywords

Shiga-Toxigenic Escherichia ColiQ1

Virulence FactorQ1

CheeseQ3

Multiplex Polymerase Chain ReactionQ2

Abstract

Introduction: Shiga toxin-producing Escherichia coli (STEC) strains are one of the main causes of food poisoning and diseases such as hemorrhagic colitis and hemolytic uremic syndrome. Food contamination mainly occurs through food of animal origin such as meat and dairy products. The aim of this study was to investigate prevalence and distribution of virulence genes in Escherichia coli isolates from traditional Cheese from Semnan city, using multiplex PCR method. Materials and Methods: In this study, a total of 100 traditional Cheese samples were screened for E. coli strains through conventional microbiological tests. Then, screening for virulence genes (Stx1, Stx2, eaeA and hlyA genes) was carried out by using PCR method. Results: Fifty-nine Escherichia coli strains were identified among 100 Cheese samples. Of these, 55 isolates exhibited for one or more virulence genes (93. 2%) which, 44 isolates carrying more than of one gene and 11 isolates were positive for the carrier of one gene. Stx2 showed the highest frequency of virulence gene in E. coli isolates. Conclusion: Therefore, due to the high prevalence of virulence genes in pathogenic E. coli isolates in traditional Cheese, it is necessary to use molecular methods to control the microbial quality of these products.

Cites

No record.

References

No record.

Cite

APA: Copy

malekiparvari, Maryam, Parsaeimehr, Mahnoosh, STAJI, HAMID, & Jebellijavan, ashkan. (2021). Molecular investigation of the prevalence and detection of enterohemorrhagic Escherichia coli isolated from traditional cheese in Iran. KOOMESH, 23(1 (81) ), 174-179. SID. https://sid.ir/paper/409180/en

Vancouver: Copy

malekiparvari Maryam, Parsaeimehr Mahnoosh, STAJI HAMID, Jebellijavan ashkan. Molecular investigation of the prevalence and detection of enterohemorrhagic Escherichia coli isolated from traditional cheese in Iran. KOOMESH[Internet]. 2021;23(1 (81) ):174-179. Available from: https://sid.ir/paper/409180/en

IEEE: Copy

Maryam malekiparvari, Mahnoosh Parsaeimehr, HAMID STAJI, and ashkan Jebellijavan, “Molecular investigation of the prevalence and detection of enterohemorrhagic Escherichia coli isolated from traditional cheese in Iran,” KOOMESH, vol. 23, no. 1 (81) , pp. 174–179, 2021, [Online]. Available: https://sid.ir/paper/409180/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops