DETECTION OF MYCOPLASMAL DNA IN RHEUMATOID ARTHRITIS PATIENTS SERUM BY PCR

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SHAH HOSSEINI M.H.; GHARIB DOUST F.; ALLAHYARY E.; KHORAM KHOURSHID H.R.; VAND YUSEFI J.

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Journal: Journal of Medical Council of Iran Year:2007 | Volume:25 | Issue:2 Page(s): 178-191

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Title

DETECTION OF MYCOPLASMAL DNA IN RHEUMATOID ARTHRITIS PATIENTS SERUM BY PCR

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Keywords

RHEUMATOID ARTHRITISQ3

PCRQ3

MYCOPLASMA SPP.Q3

Abstract

Background: Mycoplasmas have been incriminated as causes of RHEUMATOID ARTHRITIS (RA) in many animal species. In contrast, the role of mycoplasmas in human RA is controversial. Numerous attempts using conventional methods for the detection of mycoplasmas in RA have not yielded suitable results for setting relationships between mycoplasmas and RA. The advent of molecular techniques, particularly the use of the polymerse chain reaction (PCR), has brought new insights for the accurate diagnosis of mycoplasma DNA and RNA in RA samples. To search for evidence that mycoplasmas are involved in the pathogenesis of RA, we use PCR techniques for detecting the possible presence of mycoplasmas in the bloodstream of patients with RA. Methods: A highly sensitive, broad range PCR protocol using SHAH-GPO-3, MGSO primers and standard mycoplasma species, targeting the bacterial 16S rRNA gene, was designed and applied to Anti-CCP positive, negative, healthy control and RA patients’ sera. Amplicon (272 bp) was cloned by PCR-cloning and then was sequenced by dideoxy chain termination. Findings: The MYCOPLASMA SPP. sequence, on the basis 16S rRNA, was detected in 12/100 (%12) serum of Anti-CCP positive, 4/100 (%4) of Anti-CCP negative, 1/100 (%1) of healthy controls and 10/91 (%11) serum of RA patients. Using species-specific primers, we were able to detect infections with all of mycoplasma genus in RA patients. Conclusion: These findings suggest an association between mycoplasmal infection and RHEUMATOID ARTHRITIS in some patients. However, further research is required to determine the role of these organisms in the pathogenesis of RA.

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APA: Copy

SHAH HOSSEINI, M.H., GHARIB DOUST, F., ALLAHYARY, E., KHORAM KHOURSHID, H.R., & VAND YUSEFI, J.. (2007). DETECTION OF MYCOPLASMAL DNA IN RHEUMATOID ARTHRITIS PATIENTS SERUM BY PCR. JOURNAL OF MEDICAL COUNCIL OF I.R.I., 25(2), 178-191. SID. https://sid.ir/paper/41411/en

Vancouver: Copy

SHAH HOSSEINI M.H., GHARIB DOUST F., ALLAHYARY E., KHORAM KHOURSHID H.R., VAND YUSEFI J.. DETECTION OF MYCOPLASMAL DNA IN RHEUMATOID ARTHRITIS PATIENTS SERUM BY PCR. JOURNAL OF MEDICAL COUNCIL OF I.R.I.[Internet]. 2007;25(2):178-191. Available from: https://sid.ir/paper/41411/en

IEEE: Copy

M.H. SHAH HOSSEINI, F. GHARIB DOUST, E. ALLAHYARY, H.R. KHORAM KHOURSHID, and J. VAND YUSEFI, “DETECTION OF MYCOPLASMAL DNA IN RHEUMATOID ARTHRITIS PATIENTS SERUM BY PCR,” JOURNAL OF MEDICAL COUNCIL OF I.R.I., vol. 25, no. 2, pp. 178–191, 2007, [Online]. Available: https://sid.ir/paper/41411/en

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