مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

356
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

1

Information Journal Paper

Title

Evaluation of CatSper 2 gene expression and embryonic morphological parameters in mice exposed to ethephon

Pages

  379-386

Abstract

 Introduction: Ethephon is a hormone used to accelerate the plant growth regulator processing. Mostly, after entering to the tissues, causing disturbance in reproductive system and subsequently decreased Sperm fertility potential. The aim of this study was to investigate the effect of ethephon on embryonic morphological parameters and CatSper 2 protein localization in Sperm. Materials and Methods: In this experimental study, 78 males and females (NMRI Strain) Mice randomly divided into experimental and control groups. The experimental groups received ethephon at doses of 120, 240 and 480 mg/kg as well as the positive-control group received neostigmine (0. 1 mg/kg). After treatment, the animals were sacrificed and euthanized. Sperm samples were collected from cauda epididymis for qRT-PCR and immunohistochemically evaluation and stored at-70 ° C until examination. Results: The results of morphological parameters showed that hatching percentage, viability, number and length of fetuses in experimental groups were lower than control group. There was also a decrease in the expression of CatSper 2 gene in the experimental groups compared to the control group. Sperm immunohistochemical results indicated that there was no localization for the CatSper 2 protein in the experimental groups versus the ones (p<0. 05). Conclusion: Ethephon by producing free radicals causes decreased embryonic morphological features. It also impairs fertility and reproduction by decreasing the expression of CatSper 2 gene and lack of protein localization in Sperm.

Cites

References

Cite

APA: Copy

Jahangirfard, Ramin, NAJAFI, GHOLAMREZA, SHALIZAR JALALI, ALI, AHMADI, ABBAS, & ZADEH HASHEM, ELHAM. (2021). Evaluation of CatSper 2 gene expression and embryonic morphological parameters in mice exposed to ethephon. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 28(3 ), 379-386. SID. https://sid.ir/paper/414184/en

Vancouver: Copy

Jahangirfard Ramin, NAJAFI GHOLAMREZA, SHALIZAR JALALI ALI, AHMADI ABBAS, ZADEH HASHEM ELHAM. Evaluation of CatSper 2 gene expression and embryonic morphological parameters in mice exposed to ethephon. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2021;28(3 ):379-386. Available from: https://sid.ir/paper/414184/en

IEEE: Copy

Ramin Jahangirfard, GHOLAMREZA NAJAFI, ALI SHALIZAR JALALI, ABBAS AHMADI, and ELHAM ZADEH HASHEM, “Evaluation of CatSper 2 gene expression and embryonic morphological parameters in mice exposed to ethephon,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 28, no. 3 , pp. 379–386, 2021, [Online]. Available: https://sid.ir/paper/414184/en

Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button